CDKN1B 抗体 (Internal Region)
Quick Overview for CDKN1B 抗体 (Internal Region) (ABIN185582)
抗原
See all CDKN1B 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- P27KIP1 / CDKN1B (internal)
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序列
- KRPATDDSST QNK
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交叉反应
- Cat, Cow, 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-KRPATDDSSTQNK, from the internal region of the protein sequence according to NP_004055.1.
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亚型
- IgG
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anti-P27 antibody
CDKN1B 适用: 人 WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 150-198) antibodyCDKN1B 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibodyCDKN1B 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 4B4-E6 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyKO Validated CDKN1B 适用: 人 WB, IHC, IF, IHC (p), IP, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyCDKN1B 适用: 人 IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal SX53G8 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyCDKN1B 适用: 人, 小鼠, 大鼠, 猴 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal DCS-72-F6-KIP1-769 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyCDKN1B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 1G2 unconjugated Recombinant Antibody
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应用备注
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Immunohistochemistry: Paraffin embedded Human Prostate and Spleen. Recommended concentration: 5 μg/mL.
Western Blot: Approx 26 kDa band observed in lysates of Human Prostate and of Hepatoblastoma cell line HepG2 (calculated MW of 22.1 kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature with different a
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
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别名
- CDKN1B
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背景
- CDKN1B, KIP1, cyclin-dependent kinase inhibitor 1B (p27, Kip1) , CDKN4, P27KIP1, cyclin-dependent kinase inhibitor 1B
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基因ID
- 1027
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NCBI登录号
- NP_004055
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途径
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
抗原
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