Patched 1 抗体 (C-Term)
Quick Overview for Patched 1 抗体 (C-Term) (ABIN185521)
抗原
See all Patched 1 (PTCH1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
 - C-Term
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原理
 - PTCH (C Terminus)
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序列
 - ELQDVECEER PR
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特异性
 - This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1) encode the same isoform.
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交叉反应
 - 人, 小鼠, 大鼠
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纯化方法
 - Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
 - Peptide with sequence ELQDVECEERPR, from the C Terminus of the protein sequence according to NP_000255.1, NP_001077072.1, NP_001077071.1, NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1.
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亚型
 - IgG
 
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应用备注
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Immunohistochemistry: Paraffin embedded Human Brain (Coretx). Recommended concentration: 8 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:16000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the Golgi of HeLa and NIH3T3 cells. Recommended concentration: 10μg/ml.
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限制
 - 仅限研究用
 
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状态
 - Liquid
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浓度
 - 0.5 mg/mL
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缓冲液
 - Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
 - Sodium azide
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注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
 - Minimize freezing and thawing.
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储存条件
 - -20 °C
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储存方法
 - Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
 
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                                            : "Expression of Hedgehog ligand and signal transduction components in mutually distinct isocitrate dehydrogenase mutant glioma cells supports a role for paracrine signaling."    in: Journal of neuro-oncology, Vol. 119, Issue 2, pp. 243-51, (2014)         (PubMed).        
: "Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation." in: Cancer letters, Vol. 328, Issue 2, pp. 297-306, (2012) (PubMed).
 
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                                            : "Expression of Hedgehog ligand and signal transduction components in mutually distinct isocitrate dehydrogenase mutant glioma cells supports a role for paracrine signaling."    in: Journal of neuro-oncology, Vol. 119, Issue 2, pp. 243-51, (2014)         (PubMed).        
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- Patched 1 (PTCH1)
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别名
 - PTCH1
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背景
 - PTCH1, PTCH, patched homolog (Drosophila), HGNC:9585, BCNS, HPE7, NBCCS, PTC, PTC1, patched, patched (Drosophila) homolog, patched homolog 1 (Drosophila), RP11-435O5.3, FLJ26746, FLJ42602, PTCH11, OTTHUMP00000021709, OTTHUMP00000021710, PTCH protein +12b,
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基因ID
 - 5727
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NCBI登录号
 - NP_000255, NP_001077072, NP_001077071, NP_001077073, NP_001077074, NP_001077075, NP_001077076
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途径
 - Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation
 
抗原
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