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Patched 1 抗体 (C-Term)

This anti-Patched 1 antibody is a 山羊 多克隆 antibody detecting Patched 1 in ELISA, IHC 和 IF. Suitable for 人 和 小鼠. This Primary Antibody has been cited in 2+ publications.
产品编号 ABIN185521
发货至: 中国

Quick Overview for Patched 1 抗体 (C-Term) (ABIN185521)

抗原

See all Patched 1 (PTCH1) 抗体
Patched 1 (PTCH1)

适用

  • 40
  • 27
  • 9
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
人, 小鼠

宿主

  • 38
  • 7
  • 3
山羊

克隆类型

  • 42
  • 6
多克隆

标记

  • 36
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Patched 1 antibody is un-conjugated

应用范围

  • 35
  • 23
  • 19
  • 10
  • 8
  • 6
  • 5
  • 1
ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    原理

    PTCH (C Terminus)

    序列

    ELQDVECEER PR

    特异性

    This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1) encode the same isoform.

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence ELQDVECEERPR, from the C Terminus of the protein sequence according to NP_000255.1, NP_001077072.1, NP_001077071.1, NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Brain (Coretx). Recommended concentration: 8 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the Golgi of HeLa and NIH3T3 cells. Recommended concentration: 10μg/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Abiria, Williams, Munden, Grover, Wallace, Lundberg, Valadez, Cooper: "Expression of Hedgehog ligand and signal transduction components in mutually distinct isocitrate dehydrogenase mutant glioma cells supports a role for paracrine signaling." in: Journal of neuro-oncology, Vol. 119, Issue 2, pp. 243-51, (2014) (PubMed).

    Gerardo Valadez, Grover, Carter, Calcutt, Abiria, Lundberg, Williams, Cooper: "Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation." in: Cancer letters, Vol. 328, Issue 2, pp. 297-306, (2012) (PubMed).

  • 抗原

    Patched 1 (PTCH1)

    别名

    PTCH1

    背景

    PTCH1, PTCH, patched homolog (Drosophila), HGNC:9585, BCNS, HPE7, NBCCS, PTC, PTC1, patched, patched (Drosophila) homolog, patched homolog 1 (Drosophila), RP11-435O5.3, FLJ26746, FLJ42602, PTCH11, OTTHUMP00000021709, OTTHUMP00000021710, PTCH protein +12b,

    基因ID

    5727

    NCBI登录号

    NP_000255, NP_001077072, NP_001077071, NP_001077073, NP_001077074, NP_001077075, NP_001077076

    途径

    Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation
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