KPNB1 抗体 (C-Term)
Quick Overview for KPNB1 抗体 (C-Term) (ABIN185516)
抗原
See all KPNB1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
-
-
抗原表位
- C-Term
-
原理
- Karyopherin (importin) beta 1
-
序列
- RRSKTNKAKT LAT
-
交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
-
纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
免疫原
- Peptide with sequence C-RRSKTNKAKTLAT, from the C Terminus of the protein sequence according to NP_002256.2.
-
亚型
- IgG
-
-
-
-
应用备注
-
Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.
Western Blot: Approx 90-95- kDa band observed in lysates of cell lines A431, Daudi, HEK293, HeLa, Jurkat, Kelly and KNRK (calculated MW of 97.2 kDa according to Human NP_002256.2 and Rat NP_058759.2). Recommended concentration: 0.01-0.1 μg/mL. Primary incu
Peptide ELISA: antibody detection limit dilution 1:32000.
-
说明
-
Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.5 mg/mL
-
缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Minimize freezing and thawing.
-
储存条件
- -20 °C
-
储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
-
- KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))
-
别名
- KPNB1
-
背景
- KPNB1, karyopherin (importin) beta 1, HGNC:6400, IMB1, IPOB, Impnb, MGC2155, MGC2156, MGC2157, NTF97, importin 90, importin beta-1 subunit, karyopherin beta 1, nuclear factor p97, KapB1, IPO1
-
基因ID
- 3837, 16211, 24917
-
NCBI登录号
- NP_002256
-
途径
- Protein targeting to Nucleus
抗原
-