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Complement Factor H 抗体 (Internal Region)

Cited in 1 publication. This 山羊 多克隆 anti-Complement Factor H antibody specifically detects Complement Factor H in WB, IHC, ELISA 和 FACS. The antibody is reactive with 人 samples.
产品编号 ABIN185437
发货至: 中国
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Quick Overview for Complement Factor H 抗体 (Internal Region) (ABIN185437)

抗原

See all Complement Factor H (CFH) 抗体
Complement Factor H (CFH)

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This Complement Factor H antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    Complement factor H

    序列

    HLVPDRKKDQ YK

    特异性

    This antibody is expected to recognize isoform a (NP_000177.2) only.

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-HLVPDRKKDQYK, from the internal region of the protein sequence according to NP_000177.2.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Liver. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 150 kDa band observed in Human Kidney lysates and in lysates of cell line K562 (calculated MW of 139 kDa according to NP_000177.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Fujisawa, Yasumoto, Kato, Sugawara, Yoshida, Yatomi, Nangaku: "The role of anti-complement factor H antibodies in the development of atypical haemolytic uremic syndrome: a possible contribution to abnormality of platelet function." in: British journal of haematology, Vol. 189, Issue 1, pp. 182-186, (2020) (PubMed).

  • 抗原

    Complement Factor H (CFH)

    别名

    CFH

    背景

    Complement factor H , HGNC:4883, CFH, CFHL3, FHL1, HF, HF1, HF2, HUS, H factor 1 (complement), H factor 2 (complement), H factor-1 (complement), factor H-like 1, AHUS1, ARMD4, ARMS1, FH, age-related maculopathy susceptibility 1, beta-1-H-globulin, beta-1H

    基因ID

    3075

    NCBI登录号

    NP_000177

    途径

    Complement System, Cellular Response to Molecule of Bacterial Origin
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