Complement Factor H 抗体 (Internal Region)
Quick Overview for Complement Factor H 抗体 (Internal Region) (ABIN185437)
抗原
See all Complement Factor H (CFH) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- Complement factor H
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序列
- HLVPDRKKDQ YK
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特异性
- This antibody is expected to recognize isoform a (NP_000177.2) only.
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-HLVPDRKKDQYK, from the internal region of the protein sequence according to NP_000177.2.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Liver. Recommended concentration: 5 μg/mL.
Western Blot: Approx 150 kDa band observed in Human Kidney lysates and in lysates of cell line K562 (calculated MW of 139 kDa according to NP_000177.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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The role of anti-complement factor H antibodies in the development of atypical haemolytic uremic syndrome: a possible contribution to abnormality of platelet function." in: British journal of haematology, Vol. 189, Issue 1, pp. 182-186, (2020) (PubMed).
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The role of anti-complement factor H antibodies in the development of atypical haemolytic uremic syndrome: a possible contribution to abnormality of platelet function." in: British journal of haematology, Vol. 189, Issue 1, pp. 182-186, (2020) (PubMed).
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- Complement Factor H (CFH)
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别名
- CFH
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背景
- Complement factor H , HGNC:4883, CFH, CFHL3, FHL1, HF, HF1, HF2, HUS, H factor 1 (complement), H factor 2 (complement), H factor-1 (complement), factor H-like 1, AHUS1, ARMD4, ARMS1, FH, age-related maculopathy susceptibility 1, beta-1-H-globulin, beta-1H
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基因ID
- 3075
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NCBI登录号
- NP_000177
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途径
- Complement System, Cellular Response to Molecule of Bacterial Origin
抗原
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