PAI1 抗体 (Internal Region)
Quick Overview for PAI1 抗体 (Internal Region) (ABIN185384)
抗原
See all PAI1 (SERPINE1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- PAI1 / SERPINE1
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序列
- GFKIDDKGMA PALRH
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交叉反应
- 犬, 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-GFKIDDKGMAPALRH, from the internal region of the protein sequence according to NP_000593.1, NP_001158885.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Recommended concentration: 4-6 μg/mL. A customer successfully used this product on Human Breast cancer.
Western Blot: Approx 48 kDa band observed in Human Liver and HeLa lysates, while approx. 45 kDa observed in lyates of cell lines A549 and HepG2 (calculated MW of 45.1 kDa according to NP_000593.1). This molecular weight is routinely observed by other source
Peptide ELISA: antibody detection limit dilution 1:64000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Bacterial expression, purification and angiogenesis-promoting activity of human thymosin ?4." in: Protein expression and purification, Vol. 90, Issue 2, pp. 142-52, (2013) (PubMed).
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: "Bacterial expression, purification and angiogenesis-promoting activity of human thymosin ?4." in: Protein expression and purification, Vol. 90, Issue 2, pp. 142-52, (2013) (PubMed).
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- PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))
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别名
- SERPINE1
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背景
- SERPINE1, PAI1, PAI, PAI-1, PLANH1, serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, plasminogen activator inhibitor, type I, serpin peptidase inhibitor, clade E (nexin, plasminogen activator in
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分子量
- 45.1kDa
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基因ID
- 5054
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NCBI登录号
- NP_000593
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途径
- p53 Pathway, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
抗原
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