MYD88 抗体 (Internal Region)
Quick Overview for MYD88 抗体 (Internal Region) (ABIN185362)
抗原
See all MYD88 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- MYD88
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序列
- IKYKAMKKEF P
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特异性
- This antibody is expected to recognize reported isoforms 1, 2, 3 and 9.
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交叉反应
- 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-IKYKAMKKEFP, from the internal region of the protein sequence according to NP_002459.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 4-6 μg/mL.
Western Blot: Approx 28 kDa band observed in Human Spleen lysates (calculated MW of 28.3 kDa according to NP_001166039.2). Recommended concentration: 0.3-0.5 μg/mL. Primary incubation1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Immunofluorescence: Strong expression of the protein seen in U2OS and Jurkat cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))
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别名
- MYD88
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背景
- MYD88, myeloid differentiation primary response gene (88), MYD88D, myeloid differentiation primary response gene 88
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分子量
- 33.2kDa
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基因ID
- 4615, 17874, 301059
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NCBI登录号
- NP_001166038, NP_002459, NP_001166039
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途径
- NF-kappaB Signaling, TLR signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades
抗原
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