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Smooth Muscle Actin 抗体 (N-Term)

Name: Smooth Muscle Actin 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN185271
Availability: OutOfStock

This anti-Smooth Muscle Actin antibody is a 山羊多克隆 antibody detecting Smooth Muscle Actin in WB 和 ELISA. Suitable for 人, 大鼠和小鼠. This Primary Antibody has been cited in 5+ publications.

ABIN185271 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody. (Smooth Muscle Actin 抗体 (N-Term))

IHC

(ABIN185271) (1 μg/mL) staining of Human Heart (A), (0. (Smooth Muscle Actin 抗体 (N-Term))

ABIN185271 staining (1. (Smooth Muscle Actin 抗体 (N-Term))

ABIN185271 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0. (Smooth Muscle Actin 抗体 (N-Term))

ABIN185271 (0. (Smooth Muscle Actin 抗体 (N-Term))

ABIN185271 (3. (Smooth Muscle Actin 抗体 (N-Term))

IHC

ABIN185271 (1µg/ml) staining of Rat Heart lysate (35µg protein in RIPA buffer). (Smooth Muscle Actin 抗体 (N-Term))

ABIN185271 (2. (Smooth Muscle Actin 抗体 (N-Term))

IHC

(ABIN185271) (1 μg/mL) staining of HeLa (A) and NIH3T3 (B) cell lysate (35 μg protein in RIPA buffer). (Smooth Muscle Actin 抗体 (N-Term))

(ABIN185271) (1 μg/mL) staining of HeLa (A) and (2 μg/mL) NIH3T3 (B) cell lysate (35 μg protein in RIPA buffer). (Smooth Muscle Actin 抗体 (N-Term))

(ABIN185271) (1 μg/mL) staining of Mouse Duodenum lysate (35 μg protein in RIPA buffer). (Smooth Muscle Actin 抗体 (N-Term))

(ABIN185271) (1 μg/mL) staining of Human Duodenum lysate (35 μg protein in RIPA buffer). (Smooth Muscle Actin 抗体 (N-Term))

29 images

(5 references)

产品编号 ABIN185271

发货至: 中国

Quick Overview for Smooth Muscle Actin 抗体 (N-Term) (ABIN185271)

抗原

See all Smooth Muscle Actin (ACTA2) 抗体

Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

适用

人, 大鼠, 小鼠

宿主

山羊

克隆类型

多克隆

标记

This Smooth Muscle Actin antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA

质量等级

Verified

抗原表位
44

17

5

2

2

1

1

1

1

1

1

1
N-Term

原理

Smooth muscle alpha-actin

序列

EEEDSTALVC

特异性

Variants NP_001604.1 and NP_001135417.1 encode the same protein.

交叉反应

犬, 人, 小鼠, 大鼠

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

免疫原

Peptide with sequence EEEDSTALVC, from the N Terminus of the protein sequence according to NP_001604.1, NP_001135417.1.

亚型

IgG
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibody
ACTA2 适用: 人, 大鼠, 小鼠, Cow WB, IHC, IF, ELISA, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibody
KO Validated ACTA2 适用: 人 WB, IF, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibody
ACTA2 适用: 人, 大鼠 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal 1A4-ACTA2-791 unconjugated

anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 1-124) antibody
ACTA2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 2-124) antibody
ACTA2 适用: 人, 大鼠, 小鼠 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 5C1H11 unconjugated

anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 167-377) antibody
ACTA2 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody
ACTA2 适用: 人, 大鼠, 小鼠, 小鸡, 兔, 山羊 WB, IHC, ELISA, IHC (p), ICC, IHC (fro), IEM 宿主: 小鼠 Monoclonal alpha-SM1-1A4 unconjugated

anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 1-377) antibody
ACTA2 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 4A8-2H3 unconjugated

anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody
ACTA2 适用: 人, 猴 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4F4 unconjugated

anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody
ACTA2 适用: 人, 大鼠, 小鼠, 猴 WB, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 1H8 unconjugated

应用备注

Western Blot: Approx 42 kDa band observed in Human Heart, Mouse Duodenum and in lysates of cell lines HeLa and NIH3T3, and approx. 45 kDa in Rat Heart lysates (calculated MW of 42 kDa according to Human NP_001604, Mouse NP_031418.1 and Rat NP_112266.1). R
Peptide ELISA: antibody detection limit dilution 1:32000.

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Cunha, Leuckx, Sterkendries, Korf, Bomfim-Ferreira, Overbergh, Vaes, Heimberg, Gysemans, Mathieu: "Human multipotent adult progenitor cells enhance islet function and revascularisation when co-transplanted as a composite pellet in a mouse model of diabetes." in: Diabetologia, Vol. 60, Issue 1, pp. 134-142, (2017) (PubMed).

Nischalke, Berger, Lutz, Langhans, Wolter, Eisenhardt, Krämer, Kokordelis, Glässner, Müller, Rosendahl, Fischer, Berg, Grünhage, Leifeld, Soyka, Nattermann, Sauerbruch, Stickel, Spengler: "Influence of the CXCL1 rs4074 A allele on alcohol induced cirrhosis and HCC in patients of European descent." in: PLoS ONE, Vol. 8, Issue 11, pp. e80848, (2014) (PubMed).

Park, Chen, Hoffman, Marsh, West, Grunig: "Modification of hemodynamic and immune responses to exposure with a weak antigen by the expression of a hypomorphic BMPR2 gene." in: PLoS ONE, Vol. 8, Issue 1, pp. e55180, (2013) (PubMed).

Trokovic, Pöllänen, Porola, Stegaev, Hetzel, Tivesten, Engdahl, Carlsten, Forsblad-dElia, Fagman, Lagerquist, Konttinen: "Exosomal secretion of death bullets: a new way of apoptotic escape?" in: American journal of physiology. Endocrinology and metabolism, Vol. 303, Issue 8, pp. E1015-24, (2012) (PubMed).

McIntire, Sifers, Platt, Ganacias, Langat, Hunt: "Novel HLA-G-binding leukocyte immunoglobulin-like receptor (LILR) expression patterns in human placentas and umbilical cords." in: Placenta, Vol. 29, Issue 7, pp. 631-8, (2008) (PubMed).
抗原

Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

别名

ACTA2

背景

ACTA2, actin, alpha 2, smooth muscle, aorta, OTTHUMP00000020042, ACTSA, AAT6, ACTSA, alpha 2 actin, alpha-cardiac actin, growth-inhibiting gene 46, smooth muscle alpha-actin,

基因ID

59

NCBI登录号

NP_001604, NP_001135417

途径

Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development