DPF1 抗体 (C-Term)
Quick Overview for DPF1 抗体 (C-Term) (ABIN184922)
抗原
See all DPF1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
-
-
抗原表位
- C-Term
-
原理
- Neuro-d4 / DPF1
-
序列
- HLKEKASAYI TLT
-
特异性
- This antibody is expected to recognize all three reported isoforms (NP_001128627.1, NP_004638.2, NP_001128628.1).
-
交叉反应
- Cow, 人, 小鼠, 大鼠
-
纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
免疫原
- Peptide with sequence C-HLKEKASAYITLT, from the C Terminus of the protein sequence according to NP_001128627.1, NP_004638.2, NP_001128628.1.
-
亚型
- IgG
-
-
anti-D4, Zinc and Double PHD Fingers Family 1 (DPF1) (AA 261-380) antibody
DPF1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-D4, Zinc and Double PHD Fingers Family 1 (DPF1) antibodyDPF1 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-D4, Zinc and Double PHD Fingers Family 1 (DPF1) (AA 229-278) antibodyDPF1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-D4, Zinc and Double PHD Fingers Family 1 (DPF1) (Middle Region) antibodyDPF1 适用: 人, 小鼠, 大鼠, Cow, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-D4, Zinc and Double PHD Fingers Family 1 (DPF1) (Middle Region) antibodyDPF1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western Blot: Preliminary experiments showed a band at approx 38-40 kDa in Human Cerebellum and Frontal Cortex lysates after 0.3-1 μg/mL antibody staining (calculated MW of 37.9 kDa according to NP_001128628.1. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:2000.
-
说明
-
Immunofluorescence: Strong expression of the protein seen in the nuclei and weaker staining in the cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.5 mg/mL
-
缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Minimize freezing and thawing.
-
储存条件
- -20 °C
-
储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
-
- DPF1 (D4, Zinc and Double PHD Fingers Family 1 (DPF1))
-
别名
- DPF1
-
背景
- DPF1, NEUD4, neuro-d4, D4, zinc and double PHD fingers family 1, Neuro-d4 (rat) homolog, D4, zinc and double PHD fingers, family 1, MGC150428, MGC150429, BAF45b, OTTHUMP00000211615, neuro-d4 homolog
-
基因ID
- 8193, 29861, 50545
-
NCBI登录号
- NP_001128627, NP_004638, NP_001128628
抗原
-
