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JUP 抗体 (C-Term)

This anti-JUP antibody is a 山羊 多克隆 antibody detecting JUP in WB, IHC 和 ELISA. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN184775
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for JUP 抗体 (C-Term) (ABIN184775)

抗原

See all JUP 抗体
JUP (Junction Plakoglobin (JUP))

适用

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人, 小鼠, 大鼠

宿主

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山羊

克隆类型

  • 100
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多克隆

标记

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This JUP antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    Plakoglobin / Gamma-catenin

    序列

    GLRPPYPTAD HMLA

    特异性

    This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.

    交叉反应

    犬, 人, 小鼠, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-GLRPPYPTADHMLA, from the C Terminus of the protein sequence according to NP_002221.1, NP_068831.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 80 kDa band observed in lysates of cell lines A431 and NIH3T3 and in Rat Eye lysates (calculated MW of 81.7 kDa according to Human NP_002221.1 and Mouse NP_034723.1). Recommended concentration: 0.1-0.3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    JUP (Junction Plakoglobin (JUP))

    别名

    JUP

    背景

    JUP, GAMMA-CATENIN, gamma-catenin, junction plakoglobin, DP3, PDGB, PKGB, DPIII, ARVD12, CTNNG, OTTHUMP00000164735, catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80 kDa

    基因ID

    3728, 16480

    NCBI登录号

    NP_002221, NP_068831

    途径

    Cell-Cell Junction Organization, Maintenance of Protein Location
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