JUP 抗体 (C-Term)
Quick Overview for JUP 抗体 (C-Term) (ABIN184775)
抗原
See all JUP 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- Plakoglobin / Gamma-catenin
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序列
- GLRPPYPTAD HMLA
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特异性
- This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.
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交叉反应
- 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-GLRPPYPTADHMLA, from the C Terminus of the protein sequence according to NP_002221.1, NP_068831.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.
Western Blot: Approx 80 kDa band observed in lysates of cell lines A431 and NIH3T3 and in Rat Eye lysates (calculated MW of 81.7 kDa according to Human NP_002221.1 and Mouse NP_034723.1). Recommended concentration: 0.1-0.3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- JUP (Junction Plakoglobin (JUP))
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别名
- JUP
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背景
- JUP, GAMMA-CATENIN, gamma-catenin, junction plakoglobin, DP3, PDGB, PKGB, DPIII, ARVD12, CTNNG, OTTHUMP00000164735, catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80 kDa
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基因ID
- 3728, 16480
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NCBI登录号
- NP_002221, NP_068831
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途径
- Cell-Cell Junction Organization, Maintenance of Protein Location
抗原
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