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APEX1 抗体 (N-Term)

Verified APEX1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN184704
发货至: 中国
  • 抗原 See all APEX1 抗体
    APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
    抗原表位
    • 7
    • 7
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    适用
    • 85
    • 38
    • 35
    • 8
    • 7
    • 7
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    • 6
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    宿主
    • 69
    • 18
    • 1
    山羊
    克隆类型
    • 70
    • 18
    多克隆
    标记
    • 65
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This APEX1 antibody is un-conjugated
    应用范围
    • 81
    • 29
    • 29
    • 25
    • 19
    • 13
    • 7
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    原理
    APE1 / APEX1
    序列
    PKRGKKGAVA EDGD
    特异性
    Reported variants represent identical protein (NP_001632.2, NP_542379.1, NP_542380.1).
    交叉反应
    Cow, 犬, 人, Pig
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence PKRGKKGAVAEDGD-C, from the N Terminus of the protein sequence according to NP_001632.2.
    亚型
    IgG
    Top Product
    Discover our top product APEX1 Primary Antibody
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining of lobular epithelial cells. Recommended concentration: 4-6 μg/mL.

    Western Blot: Approx 37 kDa band observed in nuclear lysates of cell lines A431, HeLa and MCF7 (calculated MW of 35.6 kDa according to NP_001632.2, NP_542379.1 and NP_542380.1). Recommended concentration: 0.1-0.3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
    别名
    APEX1 (APEX1 产品)
    别名
    apex1 antibody, MGC76280 antibody, MGC79645 antibody, MGC78928 antibody, APE antibody, APE1 antibody, APEN antibody, APEX antibody, APX antibody, HAP1 antibody, REF1 antibody, Apex antibody, REF-1 antibody, BAP1 antibody, apex1a antibody, hm:zehn1532 antibody, wu:fc13d11 antibody, zgc:66204 antibody, Ref-1 antibody, apurinic/apyrimidinic endodeoxyribonuclease 1 antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog antibody, DNA-(apurinic or apyrimidinic site) lyase antibody, apurinic/apyrimidinic endonuclease 1 antibody, APEX1 antibody, apex1 antibody, apex1.L antibody, CNG03640 antibody, CNH01280 antibody, nth antibody, Spirs_4119 antibody, Palpr_0103 antibody, Apex1 antibody
    背景
    APE1, APEX1, APEX, APEX nuclease (multifunctional DNA repair enzyme), APE, APX, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, apurinic/apyrimidinic exonuclease, multifunctional DNA repair enzyme, DNA-(apurinic or apyrimidinic site) lyase, a
    基因ID
    328
    NCBI登录号
    NP_001632
    途径
    DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
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