ITK 抗体 (C-Term)
Quick Overview for ITK 抗体 (C-Term) (ABIN184605)
抗原
See all ITK 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- ITK
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序列
- RLLRQLAEIA ESGL
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交叉反应
- 人, 小鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-RLLRQLAEIAESGL, from the C Terminus of the protein sequence according to NP_005537.3.
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亚型
- IgG
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anti-IL2-Inducible T-Cell Kinase (ITK) (Internal Region) antibody
Verified ITK 适用: 人, 大鼠 WB, ELISA, IHC, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-IL2-Inducible T-Cell Kinase (ITK) antibodyITK 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 5G6 unconjugated
anti-IL2-Inducible T-Cell Kinase (ITK) (AA 2-110) antibodyITK 适用: 人 ELISA 宿主: 小鼠 Monoclonal 5G12C4 unconjugated
anti-IL2-Inducible T-Cell Kinase (ITK) (pTyr512) antibodyITK 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-IL2-Inducible T-Cell Kinase (ITK) (AA 368-625) antibodyITK 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-IL2-Inducible T-Cell Kinase (ITK) antibodyITK 适用: 人 ELISA, IHC 宿主: 兔 Monoclonal 8F10 unconjugated Recombinant Antibody
anti-IL2-Inducible T-Cell Kinase (ITK) (AA 228-262) antibodyITK 适用: 人 WB 宿主: 兔 Polyclonal RB50773 unconjugated
anti-IL2-Inducible T-Cell Kinase (ITK) (AA 1-111) antibodyITK 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-IL2-Inducible T-Cell Kinase (ITK) antibodyITK 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-IL2-Inducible T-Cell Kinase (ITK) (AA 156-190), (N-Term) antibodyITK 适用: 小鼠 WB 宿主: 兔 Polyclonal RB50676 unconjugated
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应用备注
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Western Blot: Approx 75 kDa band observed in nuclear ysates of cell line Jurkat (calculated MW of 71.8 kDa according to NP_005537.3). Recommended concentration: 1-3g/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:2000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and Jurkat cells. Recommended concentration: 10μg/ml.
Flow Cytometry: A customer reported weak staining of Jurkat cells at a concentrat
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- ITK (IL2-Inducible T-Cell Kinase (ITK))
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别名
- ITK
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背景
- ITK, EMT, LYK, PSCTK2, IL2-inducible T-cell kinase, tyrosine-protein kinase LYK, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, MGC126257, MGC126258
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基因ID
- 3702, 16428
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NCBI登录号
- NP_005537
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway
抗原
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