DLL4 抗体
Quick Overview for DLL4 抗体 (ABIN6796404)
抗原
See all DLL4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes mouse Delta-like protein 4 (DLL4), one of the five major ligands of the Notch signalling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. DLL4 is expressed by vascular endothelium, and plays a vital role in embryonic vascular development. DLL4 signalling has been shown to play a role in the angiogenesis of clear-cell renal tumors, and pancreatic, bladder and colonic cancer. Studies have shown that DLL4 expression in endothelium cells, can be up-regulated by vascular endothelial growth factor (VEGF) and basic-FGF, and by HIF1 alpha, and that blockade of DLL4 inhibits tumor growth by promoting non-productive angiogenesis. Clone HMD4-2 has been shown to block mouse Notch1-Fc and Notch4-Fc binding to mouse DLL4/CHO cells, and to inhibit subcutaneous tumor growth in mouse tumor models using the murine lung carcinoma cell line KLN205. Removal of Sodium Azide is recommended prior to use in functional assays.
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纯化方法
- Protein G purified
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免疫原
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Recombinant mouse DLL4.
Type of Immunogen: Recombinant protein -
亚型
- IgG
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anti-delta-Like 4 (DLL4) antibody
DLL4 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4A11G2 unconjugated
anti-delta-Like 4 (DLL4) (AA 313-439) antibodyDLL4 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 4A11F8 unconjugated
anti-delta-Like 4 (DLL4) (AA 215-331) antibodyDLL4 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-delta-Like 4 (DLL4) antibodyDLL4 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4A11F8 unconjugated
anti-delta-Like 4 (DLL4) antibodyDLL4 适用: 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-delta-Like 4 (DLL4) (Internal Region) antibodyDLL4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-delta-Like 4 (DLL4) antibodyDLL4 适用: 人 WB, EIA 宿主: 小鼠 Monoclonal 4A11F8-4A11G2 unconjugated
anti-delta-Like 4 (DLL4) (AA 44-345) antibodyDLL4 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-delta-Like 4 (DLL4) (AA 123-221) antibodyDLL4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-delta-Like 4 (DLL4) antibody (PE)DLL4 适用: 小鼠 FACS 宿主: 仓鼠 Monoclonal HMD4-2 PE
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- DLL4 (delta-Like 4 (DLL4))
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别名
- DLL4
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背景
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Name/Gene ID: DLL4
Synonyms: DLL4, Delta-like 4 protein, Delta like 4, Delta-like 4 (Drosophila), Delta-like 4 homolog, Drosophila Delta homolog 4, Notch ligand DLL4, Delta 4, Delta ligand 4, Delta-like protein 4, Delta4, Hdelta2, Notch ligand delta-2 -
基因ID
- 54567
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UniProt
- Q9NR61
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途径
- Notch Signaling
抗原
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