CYBB 抗体
Quick Overview for CYBB 抗体 (ABIN6796377)
抗原
See all CYBB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes the heavy chain of cytochrome B245, also known as gp91phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterized by severe recurrent infections. The heavy chain (gp91phox), along with the light chain (p22phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons. Clone NL7 binds amino acids 498-506 (EKDVITGLK), a cytosolic epitope on gp91phox not accessible on intact neutrophils. It recognizes the native as well as the denatured form of the protein. Clone NL7 has an inhibitory effect on NADPH oxidase activation. Research indicates that this inhibitory effect is exerted during the initial stages of oxidase activation. The antibody does not interfere with the translocation of the cytosolic subunits of NADPH oxidase to the cell membrane.
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纯化方法
- Protein G purified
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免疫原
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Cytochrome B245 solubilized in octyl-beta-glucopyranoside.
Type of Immunogen: Purified protein -
亚型
- IgG1
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anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 501-570) antibody
CYBB 适用: 人, 小鼠, 大鼠, 兔, 小鸡 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 283-570) antibodyCYBB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 283-570) antibodyCYBB 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome B-245, beta Polypeptide (CYBB) (C-Term) antibodyCYBB 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome B-245, beta Polypeptide (CYBB) antibodyCYBB 适用: 人 WB, IF, IHC (p), ICC 宿主: 小鼠 Monoclonal 54-1 unconjugated
anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 442-459), (Middle Region) antibodyCYBB 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 283-570) antibodyCYBB 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome B-245, beta Polypeptide (CYBB) (Internal Region) antibodyVerified CYBB 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 100-200) antibodyCYBB 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 416-500) antibodyCYBB 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Approved: ELISA, Flo (1:25 - 1:200), IHC, IHC-Fr, WB
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说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- CYBB (Cytochrome B-245, beta Polypeptide (CYBB))
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别名
- Cytochrome B245 Heavy Chain
抗原
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