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C5AR1 抗体 (N-Term)

This 小鼠 单克隆 antibody specifically detects C5AR1 in WB, FACS, IHC (p) 和 IHC (fro). It exhibits reactivity toward 人.
产品编号 ABIN181855
发货至: 中国
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Quick Overview for C5AR1 抗体 (N-Term) (ABIN181855)

抗原

See all C5AR1 抗体
C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This C5AR1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

P12-1
  • 抗原表位

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    AA 1-31, N-Term

    特异性

    This antibody recognises the C5a receptor (C5aR) CD88. Clone P12/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31). Clone P12/1 does not inhibit the binding of C5a to its receptor. We recommend the use of SM1182A for functional studies.

    纯化方法

    Affinity Chromatography on Protein G

    免疫原

    C5aR - peptide: Met1 - Asn31. Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.

    亚型

    IgG2a
  • 应用备注

    Western Blot. Immunohistochemistry on frozen section. Immunohistochemistry on paraffin section. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 5 x 10e5 cells in 100 μL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS containing 0.09 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

    别名

    CD88 / C5R1

    背景

    C5a Anaphylatoxin Receptor mediates the effects of one of the most potent chemotactic agents known, in a wide variety of inflammatory cell types. C5a receptor 1 has been implicated in asthma. CD88 is predominantly expressed on cells of the myeloid lineage.Synonyms: C5AR1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, Complement Component 5a Receptor 1

    基因ID

    728

    NCBI登录号

    NP_001727

    UniProt

    P21730

    途径

    Complement System
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