TGFA 抗体 (Biotin)
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Quick Overview for TGFA 抗体 (Biotin) (ABIN181752)
抗原
See all TGFA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects TGF-α.
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纯化方法
- Affinity chromatograophy
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免疫原
- Highly pure (> 98 %) recombinant human TGF-alpha
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应用备注
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ELISA: Direct: To detect hTGF-α (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTGF-α. Sandwich: To detect hTGF-α (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanTGF-α as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hTGF-α. Western blot: To detect hTGF-α this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hTGF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use.
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
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- TGFA (Transforming Growth Factor, alpha (TGFA))
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别名
- TGF-alpha (TGFA)
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背景
- Synonyms: EGF-like TGF, ETGF, TGF type 1, Transforming growth factor alpha
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基因ID
- 7039
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NCBI登录号
- NP_001093161
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UniProt
- P01135
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途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
抗原
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