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TGFA 抗体 (Biotin)

This anti-TGFA antibody is a 兔 多克隆 antibody detecting TGFA in WB 和 EIA. Suitable for 人.
产品编号 ABIN181752
发货至: 中国

Quick Overview for TGFA 抗体 (Biotin) (ABIN181752)

抗原

See all TGFA 抗体
TGFA (Transforming Growth Factor, alpha (TGFA))

适用

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宿主

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克隆类型

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多克隆

标记

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This TGFA antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 特异性

    This antibody detects TGF-α.

    纯化方法

    Affinity chromatograophy

    免疫原

    Highly pure (> 98 %) recombinant human TGF-alpha
  • 应用备注

    ELISA: Direct: To detect hTGF-α (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTGF-α. Sandwich: To detect hTGF-α (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanTGF-α as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hTGF-α. Western blot: To detect hTGF-α this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hTGF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

    缓冲液

    PBS, pH 7.2

    注意事项

    This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use.

    储存条件

    4 °C

    储存方法

    Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
  • 抗原

    TGFA (Transforming Growth Factor, alpha (TGFA))

    别名

    TGF-alpha (TGFA)

    背景

    Synonyms: EGF-like TGF, ETGF, TGF type 1, Transforming growth factor alpha

    基因ID

    7039

    NCBI登录号

    NP_001093161

    UniProt

    P01135

    途径

    NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
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