NOV 抗体
Quick Overview for NOV 抗体 (ABIN181716)
抗原
See all NOV 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects NOV.
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纯化方法
- Immunoaffinity Chromatography
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免疫原
- Highly pure (> 98%) recombinant Human NOV
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anti-Nephroblastoma Overexpressed (NOV) (Center) antibody
NOV 适用: 人, 小鼠 WB, IHC (p), ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nephroblastoma Overexpressed (NOV) (AA 334-357), (C-Term) antibodyNOV 适用: 大鼠 WB, IHC, ICC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nephroblastoma Overexpressed (NOV) (AA 1-357) antibodyNOV 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Nephroblastoma Overexpressed (NOV) (AA 32-131) antibodyNOV 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 2G8 unconjugated
anti-Nephroblastoma Overexpressed (NOV) (AA 1-357) antibodyNOV 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated
anti-Nephroblastoma Overexpressed (NOV) (AA 334-357) antibodyNOV 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nephroblastoma Overexpressed (NOV) (AA 221-320) antibodyNOV 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nephroblastoma Overexpressed (NOV) antibodyNOV 适用: 人 WB, IHC, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 2G8 unconjugated
anti-Nephroblastoma Overexpressed (NOV) (AA 156-351) antibodyNOV 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nephroblastoma Overexpressed (NOV) (AA 32-357) antibodyNOV 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Indirect ELISA: To detect hNOV by (using 100 μL/well antibody solution) a concentration of0.5-2.0 μg/mL of this antibody is required. This antibody in conjunction with compatiblesecondary reagents, it allows the detection of at least 0.2-0.4 ng/well of recombinanthNOV. Sandwich ELISA: To detect hNOV (using 100 μL/well antibody solution) a concentration of0.5-2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanNOV (ABIN181714 or ABIN181713) as a detection antibody, it allows the detection of atleast 0.2-0.4 ng/well of recombinant hNOV. Western blot: To detect hNOV this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant hNOV is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry on Formalin-Fixed, Paraffin Sections: 0.25-0.50 μg/mL with anovernight incubation at 4 °C. An HRP-labeled polymer detection system was used with aDAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer isrecommended. Positive Control: Human breast invasive ductal carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- NOV (Nephroblastoma Overexpressed (NOV))
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别名
- NOV
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背景
- CCN3 is a cysteine-rich protein that is overexpressed in avian nephroblastomas. It is a member of the CCN family of proteins that includes CTGF. These proteins are encoded by a group of genes known as immediate-early genes, so named because they are expressed after induction by growth factors or certain oncogenes. The proteins share several common structural motifs: a consensus sequence present in IGF (insulin-like growth factor)-binding proteins (the IGFBP motif), an oligomeric complex-forming domain first identified in von Willebrand factor, a binding domain to soluble and matrix molecules and a dimerization (CT) domain. All CCN family members are thought to be involved in the control of cell proliferation.Synonyms: CCN3, IGFBP9, NOVH, NovH, Protein NOV homolog
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基因ID
- 4856
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NCBI登录号
- NP_002505
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UniProt
- P48745
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途径
- Smooth Muscle Cell Migration, Growth Factor Binding
抗原
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