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NOV 抗体 (Biotin)

The Biotin-conjugated 兔 多克隆 anti-NOV antibody (ABIN181713) specifically detects NOV in WB 和 EIA. The antibody is reactive with 人 samples.
产品编号 ABIN181713
发货至: 中国
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Quick Overview for NOV 抗体 (Biotin) (ABIN181713)

抗原

See all NOV 抗体
NOV (Nephroblastoma Overexpressed (NOV))

适用

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宿主

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克隆类型

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多克隆

标记

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This NOV antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 特异性

    This antibody detects NOV.

    纯化方法

    Affinity chromatography

    免疫原

    Highly pure (> 98 %) recombinant human NOV
  • 应用备注

    ELISA: Direct: To detect hNOV (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNOV. Sandwich: To detect hNOV (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanNOV as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hNOV. Western blot: To detect hNOV this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hNOV is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

    缓冲液

    PBS, pH 7.2

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

    有效期

    6 months
  • 抗原

    NOV (Nephroblastoma Overexpressed (NOV))

    别名

    NOV

    背景

    CCN3 is a cysteine-rich protein that is overexpressed in avian nephroblastomas. It is a member of the CCN family of proteins that includes CTGF. These proteins are encoded by a group of genes known as immediate-early genes, so named because they are expressed after induction by growth factors or certain oncogenes. The proteins share several common structural motifs: a consensus sequence present in IGF (insulin-like growth factor)-binding proteins (the IGFBP motif), an oligomeric complex-forming domain first identified in von Willebrand factor, a binding domain to soluble and matrix molecules and a dimerization (CT) domain. All CCN family members are thought to be involved in the control of cell proliferation.Synonyms: CCN3, IGFBP9, NOVH, NovH, Protein NOV homolog

    基因ID

    4856

    NCBI登录号

    NP_002505

    UniProt

    P48745

    途径

    Smooth Muscle Cell Migration, Growth Factor Binding
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