NOV 抗体 (Biotin)
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Quick Overview for NOV 抗体 (Biotin) (ABIN181713)
抗原
See all NOV 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects NOV.
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纯化方法
- Affinity chromatography
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免疫原
- Highly pure (> 98 %) recombinant human NOV
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应用备注
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ELISA: Direct: To detect hNOV (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNOV. Sandwich: To detect hNOV (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanNOV as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hNOV. Western blot: To detect hNOV this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hNOV is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- NOV (Nephroblastoma Overexpressed (NOV))
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别名
- NOV
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背景
- CCN3 is a cysteine-rich protein that is overexpressed in avian nephroblastomas. It is a member of the CCN family of proteins that includes CTGF. These proteins are encoded by a group of genes known as immediate-early genes, so named because they are expressed after induction by growth factors or certain oncogenes. The proteins share several common structural motifs: a consensus sequence present in IGF (insulin-like growth factor)-binding proteins (the IGFBP motif), an oligomeric complex-forming domain first identified in von Willebrand factor, a binding domain to soluble and matrix molecules and a dimerization (CT) domain. All CCN family members are thought to be involved in the control of cell proliferation.Synonyms: CCN3, IGFBP9, NOVH, NovH, Protein NOV homolog
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基因ID
- 4856
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NCBI登录号
- NP_002505
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UniProt
- P48745
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途径
- Smooth Muscle Cell Migration, Growth Factor Binding
抗原
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