IGFBP5 抗体 (Biotin)
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- 抗原 See all IGFBP5 抗体
- IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IGFBP5 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This antibody detetcs Insulin-like Growth Factor Binding Protein 5.
- 纯化方法
- Affinity chromatography
- 免疫原
- Highly pure (> 98 %) recombinant human IGF-BP5
- Top Product
- Discover our top product IGFBP5 Primary Antibody
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- 应用备注
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ELISA: Direct: To detect hIGF-BP5 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP5. Sandwich: To detect hIGF-BP5 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanIGF-BP5 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIGF-BP5. Western blot: To detect hIGF-BP5 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hIGF-BP5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
- 缓冲液
- PBS, pH 7.2
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
- 有效期
- 6 months
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- 抗原
- IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
- 别名
- IGFBP5 (IGFBP5 产品)
- 别名
- IBP5 antibody, AI256729 antibody, AW208790 antibody, IGFBP-5 antibody, IGFBP-5P antibody, IGF-BP5 antibody, igfbp5 antibody, ibp5 antibody, IBP-5 antibody, xIGFBP-5 antibody, IGFBP5 antibody, igfbp5b antibody, zgc:165472 antibody, insulin like growth factor binding protein 5 antibody, insulin-like growth factor binding protein 5 antibody, insulin like growth factor binding protein 5 L homeolog antibody, insulin-like growth factor binding protein 5b antibody, insulin-like growth factor-binding protein 5 antibody, insulin-like growth factor binding protein 5a antibody, IGFBP5 antibody, Igfbp5 antibody, igfbp5.L antibody, igfbp5b antibody, igfbp5 antibody, LOC100194500 antibody, igfbp5a antibody
- 背景
- IGFBP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFBP5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGFBP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development.Synonyms: IBP-5, IBP5, IGF-binding protein 5, IGFBP-5, Insulin-like growth factor-binding protein 5
- 基因ID
- 3488
- NCBI登录号
- NP_000590
- UniProt
- P24593
- 途径
- WNT signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding
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