RANKL 抗体
Quick Overview for RANKL 抗体 (ABIN181556)
抗原
See all RANKL (TNFSF11) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects RANKL.
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纯化方法
- Immunoaffinity purified
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免疫原
- Highly pure (> 98 %) recombinant human sRANKL
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亚型
- IgG
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anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 221-300) antibody
TNFSF11 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 74-308) antibodyTNFSF11 适用: 人, 猴 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 8A7B9 unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 210-317) antibodyTNFSF11 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (C-Term) antibodyTNFSF11 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 92-263) antibodyTNFSF11 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 106-240) antibodyTNFSF11 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 1-30) antibodyTNFSF11 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 73-306) antibodyTNFSF11 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (AA 222-316) antibodyTNFSF11 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3F7 unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) (Internal Region) antibodyVerified TNFSF11 适用: 人, 小鼠 ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
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应用备注
- ELISA: Indirect: To detect hsRANKL by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hsRANKL. Sandwich: To detect hsRANKL by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Anti-Human sRANKL as a detection antibody, it allows the detection of at least0.2 - 0.4 ng/well of recombinant hsRANKL. Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
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限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
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别名
- CD254 / RANKL
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背景
- RANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. There are three isoforms of RANKL. Human RANKL is a soluble 20 kDa polypeptide, comprising the TNF homologous region of RANKL (176 amino acid residues). This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor associated factor (TRAF) 6, which indicated that this protein may have a role in the regulation of cell apoptosis. RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.Synonyms: ODF, OPGL, Osteoclast differentiation factor, Osteoprotegerin ligand, RANK Ligand, RANKL, Receptor activator of nuclear factor kappa B ligand, TNF-related activation-induced cytokine, TNFSF11, TRANCE, Tumor necrosis factor ligand superfamily member 11
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分子量
- Approx. 150 kDa
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基因ID
- 8600
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NCBI登录号
- NP_003692
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UniProt
- O14788
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途径
- NF-kappaB Signaling
抗原
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