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IL12A 抗体

The 小鼠 单克隆 anti-IL12A antibody is suitable to detect IL12A in samples from 人. It has been validated for WB, EIA 和 IF.
产品编号 ABIN181145
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for IL12A 抗体 (ABIN181145)

抗原

See all IL12A 抗体
IL12A (Interleukin 12 alpha (IL12A))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This IL12A antibody is un-conjugated

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA), Immunofluorescence (IF)
  • 特异性

    This antibody detects Human Interleukin-12. Other species not tested.

    产品特性

    Synonyms: IL-12A, IL-12 subunit p35, NKSF1, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDasubunit, NK cell stimulatory factor chain 1

    纯化方法

    From Ascites by Affinity Chromatography on Protein A

    免疫原

    Highly pure (>98%) recombinant Human IL-12

    亚型

    IgG1
  • 应用备注

    ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of thisantibody will detect at least 1000 pg/mL of recombinant Human IL-12 when used with Biotinanti-Human IL-12 antibody (cat. PP1102B) as the detection antibody at a concentration ofapproximately 4.0-8.0 μg/mL. Western Blot: To detect Human IL-12 by Western Blot analysis this antibody can be used ata concentration of 0.50-1.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hIL-12 is 0.5-1.0 ng/lane, under non-reducingconditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore in sterile water to a concentration of 1.0 mg/mL.

    缓冲液

    PBS without preservatives

    储存液

    Without preservative

    储存条件

    4 °C/-20 °C

    储存方法

    The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.

    有效期

    12 months
  • 抗原

    IL12A (Interleukin 12 alpha (IL12A))

    别名

    Interleukin-12 alpha / IL12A

    背景

    IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-γ production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-Gamma producing Th1 cells.Synonyms: CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, NK cell stimulatory factor chain 1, NKSF1

    基因ID

    3592

    UniProt

    P29459

    途径

    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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