Immunoprecipitation. Functional Assays. Flow Cytometry: use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
有效期
12 months
抗原
MICA
(MHC Class I Polypeptide-Related Sequence A (MICA))
MIC-A antibody, PERB11.1 antibody, MHC class I polypeptide-related sequence A antibody, MICA antibody
背景
MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumours. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells.Synonyms: MHC class I polypeptide-related sequence A, MIC-A, PERB11.1