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Thrombospondin 1 抗体 (N-Term)

This anti-Thrombospondin 1 antibody is a 山羊 多克隆 antibody detecting Thrombospondin 1 in WB, IHC, ELISA, IF 和 FACS. Suitable for 人 和 小鼠.
产品编号 ABIN1781811
发货至: 中国

Quick Overview for Thrombospondin 1 抗体 (N-Term) (ABIN1781811)

抗原

See all Thrombospondin 1 (THBS1) 抗体
Thrombospondin 1 (THBS1)

适用

  • 55
  • 30
  • 27
  • 8
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
人, 小鼠

宿主

  • 90
  • 10
  • 1
山羊

克隆类型

  • 90
  • 11
多克隆

标记

  • 59
  • 20
  • 8
  • 5
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Thrombospondin 1 antibody is un-conjugated

应用范围

  • 71
  • 51
  • 37
  • 29
  • 27
  • 10
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  • 4
  • 3
  • 2
  • 2
  • 2
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  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

    • 10
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    原理

    Thrombospondin

    序列

    NRIPESGGDN SVFD

    交叉反应

    Cow, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 150 kDa band observed in Mouse Kidney lysates (calculated MW of 129 kDa according to NP_035710.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and HepG2 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    Thrombospondin 1 (THBS1)

    别名

    THBS1

    背景

    THBS1, thrombospondin 1, THBS, TSP, TSP1, thrombospondin-1p180

    基因ID

    7057

    NCBI登录号

    NP_003237

    途径

    Autophagy
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