Thrombospondin 1 抗体 (N-Term)
Quick Overview for Thrombospondin 1 抗体 (N-Term) (ABIN1781811)
抗原
See all Thrombospondin 1 (THBS1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
 - N-Term
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原理
 - Thrombospondin
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序列
 - NRIPESGGDN SVFD
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交叉反应
 - Cow, 人, 小鼠, 大鼠
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纯化方法
 - Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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亚型
 - IgG
 
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应用备注
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Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.
Western Blot: Approx 150 kDa band observed in Mouse Kidney lysates (calculated MW of 129 kDa according to NP_035710.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and HepG2 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration
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限制
 - 仅限研究用
 
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状态
 - Liquid
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浓度
 - 0.5 mg/mL
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缓冲液
 - Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
 - Sodium azide
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注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
 - Minimize freezing and thawing.
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储存条件
 - -20 °C
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储存方法
 - Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
 
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