GFP 抗体 (CypHer5E)
Quick Overview for GFP 抗体 (CypHer5E) (ABIN1742297)
抗原
See all GFP 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Rabbit anti-GFP antibody
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产品特性
- fluorescence-labeled with Cy pH er5E. Cy pH er5E® is a pH -sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. Cy pH er5E® can be detected with the filter sets for Cy5®. Cy pH er5E®: λex 647 nm / λem 667 nm
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纯化方法
- affinity purified
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应用备注
- WB:N/A IP:N/A ICC:1 : 100 up to 1 : 1000, IHC:not tested yet IHC-P:not tested yet
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说明
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ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.
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缓冲液
- PBS
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储存液
- Without preservative
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注意事项
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
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储存条件
- -20 °C,-80 °C
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储存方法
- Albumin was added for stabilization. Reconstitute immediately upon receipt! Either add 1:1 (v/v) glycerol then aliquot and store at -20°C until use or store aliquots at -80°C without additives.
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: "How to scale down postsynaptic strength." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, (2013) (PubMed).
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: "How to scale down postsynaptic strength." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, (2013) (PubMed).
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- GFP (Green Fluorescent Protein (GFP))
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别名
- GFP
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背景
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Synonyms: Sirius, meGFP, eGFP, paGFP, acGFP, eYFP, sfGFP, Green fluorescent protein, supereclyptic pH luorin, Cerulean, eCFP, mTFP, Venus, Citrine
Antigen Information: GFP is a widely used green fluorescent protein tag
抗原
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