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GFP 抗体 (CypHer5E)

Cited in 1 publication. This anti-GFP antibody is a 兔 多克隆 antibody detecting GFP in ICC. Suitable for Aequorea victoria.
产品编号 ABIN1742297
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for GFP 抗体 (CypHer5E) (ABIN1742297)

抗原

See all GFP 抗体
GFP (Green Fluorescent Protein (GFP))

适用

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Aequorea victoria

宿主

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克隆类型

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多克隆

标记

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This GFP antibody is conjugated to CypHer5E

应用范围

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Immunocytochemistry (ICC)
  • 原理

    Rabbit anti-GFP antibody

    产品特性

    fluorescence-labeled with Cy pH er5E. Cy pH er5E® is a pH -sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. Cy pH er5E® can be detected with the filter sets for Cy5®. Cy pH er5E®: λex 647 nm / λem 667 nm

    纯化方法

    affinity purified
  • 应用备注

    WB:N/A IP:N/A ICC:1 : 100 up to 1 : 1000, IHC:not tested yet IHC-P:not tested yet

    说明

    ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

    缓冲液

    PBS

    储存液

    Without preservative

    注意事项

    Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

    储存条件

    -20 °C,-80 °C

    储存方法

    Albumin was added for stabilization. Reconstitute immediately upon receipt! Either add 1:1 (v/v) glycerol then aliquot and store at -20°C until use or store aliquots at -80°C without additives.
  • Tatavarty, Sun, Turrigiano: "How to scale down postsynaptic strength." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, (2013) (PubMed).

  • 抗原

    GFP (Green Fluorescent Protein (GFP))

    别名

    GFP

    背景

    Synonyms: Sirius, meGFP, eGFP, paGFP, acGFP, eYFP, sfGFP, Green fluorescent protein, supereclyptic pH luorin, Cerulean, eCFP, mTFP, Venus, Citrine

    Antigen Information: GFP is a widely used green fluorescent protein tag

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