GFP 抗体 (AA 183-191)
(3 references)Quick Overview for GFP 抗体 (AA 183-191) (ABIN1742292)
抗原
See all GFP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 183-191
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特异性
- Specific for GFP of jelly-fish.
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纯化方法
- purified IgG. Azide was added before lyophilization.
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免疫原
- Recombinant full length GFP, glutaraldehyde fixed. Epitope QQNTPIGDG (aa 183-191)
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亚型
- IgG2b
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anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IHC, IP, ICC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FM, FACS, DB 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FM, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1 : 1000 up to 1 : 10 000 (AP staining)
IP: no (methanol fixated material only)
IHC: not tested yet -
说明
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ABIN1742292 is recommended for IP and ICC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
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缓冲液
- PBS, 0.02% sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
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储存条件
- -20 °C
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储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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: "Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin." in: Molecular biology of the cell, Vol. 24, Issue 18, pp. 2861-75, (2013) (PubMed).
: "Microtubules as platforms for assaying actin polymerization in vivo." in: PLoS ONE, Vol. 6, Issue 5, pp. e19931, (2011) (PubMed).
: "Rab4b is a small GTPase involved in the control of the glucose transporter GLUT4 localization in adipocyte." in: PLoS ONE, Vol. 4, Issue 4, pp. e5257, (2009) (PubMed).
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: "Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin." in: Molecular biology of the cell, Vol. 24, Issue 18, pp. 2861-75, (2013) (PubMed).
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- GFP (Green Fluorescent Protein (GFP))
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别名
- GFP
抗原
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