PINK1 抗体 (AA 112-496) (Atto 488)
Quick Overview for PINK1 抗体 (AA 112-496) (Atto 488) (ABIN1741109)
抗原
See all PINK1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 112-496
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特异性
- Detects ~50 kDa.
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Protein G Purified
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免疫原
- Fusion protein amino acids 112-496 (cytoplasmic C-terminus) of human PINK1. 82% identical to rat and 81% identical to mouse. >30% identity with DMPK.
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亚型
- IgG1
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anti-PTEN Induced Putative Kinase 1 (PINK1) (Internal Region) antibody
Verified PINK1 适用: 人 WB, ELISA, FACS 宿主: 山羊 Polyclonal unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-273) antibodyPINK1 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 3A9F8 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) antibodyPINK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 小鼠 Monoclonal 38CT20-8-5 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-273) antibodyPINK1 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5F2B11 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (APC)PINK1 适用: 人 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S4-15 APC
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (PerCP)PINK1 适用: 人 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S4-15 PerCP
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibodyPINK1 适用: 人 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S4-15 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (HRP)PINK1 适用: 人 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S4-15 HRP
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (FITC)PINK1 适用: 人 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S4-15 FITC
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (Biotin)PINK1 适用: 人 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal S4-15 Biotin
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应用备注
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- WB (1:1000)
- optimal dilutions for assays should be determined by the user.
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说明
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1 μg/ml of ABIN1741109 was sufficient for detection of PINK1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- PINK1 (PTEN Induced Putative Kinase 1 (PINK1))
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别名
- PINK1
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背景
- PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins, and is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 is synthesized as a 63 kD protein which undergoes proteolyt processing to generate at least two cleaved forms (55 kD and 42 kD). PINK1 and its substrates have been found in the cytosol as well as in different sub-mitochondrial compartments, and according to the recent reports, PINK1 may be targeted to OMM (outer mitochondrial membrane) with its kinase domain facing the cytosol, providing a possible explanation for the observed physical interaction with the cytosolic E3 ubiquitin ligase Parkin. Defective PINK1 may cause alterations in processing, stability, localization and activity as well as binding to substrates/interaction-partners which ultimately leads to differential effects on mitochondrial function and morphology. Mutations in PINK1 are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of alpha-synuclein, as well as proteasome dysfunction.
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基因ID
- 65018
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NCBI登录号
- NP_115785
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UniProt
- Q9BXM7
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途径
- Autophagy
抗原
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