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GFP 抗体

This anti-GFP antibody is a 小鼠 单克隆 antibody detecting GFP in WB, IHC, IP, ICC 和 FACS. Suitable for Aequorea victoria. This Primary Antibody has been cited in 3+ publications.
产品编号 ABIN1724673
发货至: 中国

Quick Overview for GFP 抗体 (ABIN1724673)

抗原

See all GFP 抗体
GFP (Green Fluorescent Protein (GFP))

适用

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Aequorea victoria

宿主

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小鼠

克隆类型

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单克隆

标记

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This GFP antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

克隆位点

4B10B2
  • 纯化方法

    purified

    免疫原

    Purified recombinant fragment of GFP expressed in E. Coli.

    亚型

    IgG2a
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Purified antibody in PBS containing 0.03 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
  • Dittman, Kaplan: "Factors regulating the abundance and localization of synaptobrevin in the plasma membrane." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 30, pp. 11399-404, (2006) (PubMed).

    Cavalieri, Bernardo, Spinelli: "Regulatory sequences driving expression of the sea urchin Otp homeobox gene in oral ectoderm cells." in: Gene expression patterns : GEP, Vol. 7, Issue 1-2, pp. 124-30, (2006) (PubMed).

    Arasada, Son, Ramalingam, Eichinger, Schleicher, Rohlfs: "Characterization of the Ste20-like kinase Krs1 of Dictyostelium discoideum." in: European journal of cell biology, Vol. 85, Issue 9-10, pp. 1059-68, (2006) (PubMed).

  • 抗原

    GFP (Green Fluorescent Protein (GFP))

    别名

    GFP

    背景

    GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

    分子量

    27kDa

    UniProt

    P42212
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