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HSP27 抗体 (AA 1-205)

The 小鼠 单克隆 anti-HSP27 antibody (Clone 2A5) (ABIN165408) specifically detects HSP27 in WB, IHC, ELISA, IF, FACS 和 ICC. The antibody is reactive with 人 samples.
产品编号 ABIN165408
发货至: 中国
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Quick Overview for HSP27 抗体 (AA 1-205) (ABIN165408)

抗原

See all HSP27 (HSPB1) 抗体
HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This HSP27 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

克隆位点

2A5
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    AA 1-205

    原理

    Human HSP27 antibody

    纯化方法

    Purified

    免疫原

    Recombinant human Hsp27 (1-205aa) purified from E. coli

    亚型

    IgG1 kappa
  • 应用备注

    Recommended dilution 1:1000

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Phosphate-Buffered Saline ( pH 7.4) with 0.02 % Sodium Azide, 10 % glycerol

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
  • 抗原

    HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

    别名

    HSP27

    背景

    Hsp27 (also known as the Estrogen-Regulated 24k protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPkAP kinase 2 and the stress-activated protein kinase SAPk2 (p38). Hsp27 forms a multimeric complex within cells, and its primary chaperone function, like other small Hsps, appears to be the stabilization of denatured or aggregated proteins in a refolding competent state.

    NCBI登录号

    NP_001531

    途径

    MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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