MAPKAP Kinase 5 抗体 (AA 192-206)
Quick Overview for MAPKAP Kinase 5 抗体 (AA 192-206) (ABIN1590013)
抗原
See all MAPKAP Kinase 5 (MAPKAPK5) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 192-206
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原理
- MK5 / MAPKAPK5 (aa192-206)
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序列
- PQVLEAQRRH QKEKS
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交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining in the DCT. Recommended concentration, 4-8 μg/mL.
Western Blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP , only the expected band was observed in the MK5 lane after EB11928 labeling. Data obtained from Dr. M. B. Menon, Inst. Bioche
Peptide ELISA: antibody detection limit dilution 1:64000.
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说明
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Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11927 or EB11928. Western blots of
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
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别名
- Mapkapk5
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背景
- MAPKAPK5, mitogen-activated protein kinase-activated protein kinase 5, MAPKAP-K5, MK-5, MK5, PRAK, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK-5, p38-regulated/activated protein kinase
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基因ID
- 8550, 17165, 498183
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NCBI登录号
- NP_003659, NP_620777
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途径
- MAPK Pathway
抗原
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