MAPK6 抗体 (Internal Region)
Quick Overview for MAPK6 抗体 (Internal Region) (ABIN1590009)
抗原
See all MAPK6 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- ERK3 / MAPK6
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序列
- HSPVGSPLKS IQ
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特异性
- Reported variants represent identical protein (NP_056621.4, NP_081694.1).
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交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 10 μg/mL.
Western Blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11926. Western blots of the IP were
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))
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别名
- Mapk6
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背景
- MAPK6, mitogen-activated protein kinase 6, ERK3, HsT17250, PRKM6, p97MAPK, ERK-3, MAP kinase 6, MAP kinase isoform p97, MAPK 6, extracellular signal-regulated kinase 3, extracellular signal-regulated kinase, p97, p97-MAPK, protein kinase, mitogen-activate
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基因ID
- 5597, 50772, 58840
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NCBI登录号
- NP_002739
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途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C
抗原
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