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MAPK6 抗体 (Internal Region)

The 山羊 多克隆 anti-MAPK6 antibody (ABIN1590009) specifically detects MAPK6 in WB, ELISA, IHC 和 IP. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN1590009
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for MAPK6 抗体 (Internal Region) (ABIN1590009)

抗原

See all MAPK6 抗体
MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

适用

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人, 小鼠

宿主

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山羊

克隆类型

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多克隆

标记

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This MAPK6 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    ERK3 / MAPK6

    序列

    HSPVGSPLKS IQ

    特异性

    Reported variants represent identical protein (NP_056621.4, NP_081694.1).

    交叉反应

    Cow, 犬, 人, 小鼠, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 10 μg/mL.

    Western Blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11926. Western blots of the IP were

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

    别名

    Mapk6

    背景

    MAPK6, mitogen-activated protein kinase 6, ERK3, HsT17250, PRKM6, p97MAPK, ERK-3, MAP kinase 6, MAP kinase isoform p97, MAPK 6, extracellular signal-regulated kinase 3, extracellular signal-regulated kinase, p97, p97-MAPK, protein kinase, mitogen-activate

    基因ID

    5597, 50772, 58840

    NCBI登录号

    NP_002739

    途径

    MAPK Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C
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