Patched 1 抗体 (AA 266-280)
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Quick Overview for Patched 1 抗体 (AA 266-280) (ABIN1590007)
抗原
See all Patched 1 (PTCH1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 266-280
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原理
- PTCH1 (aa266-280)
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序列
- EELKKINYQV DSWEE
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特异性
- This antibody is expected to recognize all reported isoforms (NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077073.1). Reported variants represent identical protein: NP_001077075.1, NP_001077074.1, NP_001077073.1, NP_001077076.1
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交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-EELKKINYQVDSWEE, from the C or N Terminus of the protein sequence according to NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077073.1.
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亚型
- IgG
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anti-Patched 1 (PTCH1) (C-Term) antibody
Verified PTCH1 适用: 人, 小鼠 ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Patched 1 (PTCH1) (AA 1214-1312) antibodyPTCH1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Patched 1 (PTCH1) antibodyPTCH1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Patched 1 (PTCH1) (AA 213-422) antibodyPTCH1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Patched 1 (PTCH1) (AA 770-970) antibodyPTCH1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Patched 1 (PTCH1) antibodyPTCH1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Patched 1 (PTCH1) (Internal Region) antibodyVerified PTCH1 适用: 人, 小鼠 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Patched 1 (PTCH1) (N-Term) antibodyPTCH1 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Patched 1 (PTCH1) antibodyPTCH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Patched 1 (PTCH1) (C-Term) antibodyPTCH1 适用: 人, 小鼠, 大鼠, 斑马鱼, 犬, Cow, 马, 豚鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 8 μg/mL.
Western Blot: Approx 140 kDa band observed in Mouse fetal Kidney lysates (calculated MW of 159 kDa according to NP_032983.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5μg/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Patched 1 (PTCH1)
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别名
- PTCH1
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背景
- PTCH1, patched 1, BCNS, FLJ26746, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH, PTCH11, OTTHUMP00000021709, OTTHUMP00000021710, PTCH protein +12b, PTCH protein +4', PTCH protein -10, patched homolog 1, protein patched homolog 1
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基因ID
- 5727, 19206, 89830
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NCBI登录号
- NP_000255, NP_001077072, NP_001077071, NP_001077073
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途径
- Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation
抗原
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