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ITK 抗体 (Internal Region)

This 山羊 多克隆 anti-ITK antibody specifically detects ITK in WB, ELISA, IHC, FACS 和 IF. The antibody is reactive with 人 和 大鼠 samples.
产品编号 ABIN1589997
发货至: 中国
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Our Local Distributor

中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for ITK 抗体 (Internal Region) (ABIN1589997)

抗原

See all ITK 抗体
ITK (IL2-Inducible T-Cell Kinase (ITK))

适用

  • 57
  • 20
  • 12
  • 5
  • 3
人, 大鼠

宿主

  • 71
  • 5
  • 1
山羊

克隆类型

  • 61
  • 16
多克隆

标记

  • 32
  • 9
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This ITK antibody is un-conjugated

应用范围

  • 49
  • 33
  • 22
  • 14
  • 14
  • 12
  • 7
  • 4
  • 4
  • 4
  • 2
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

    • 10
    • 10
    • 7
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    原理

    ITK

    序列

    KERPEDRPAF SR

    交叉反应

    Cow, 人, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 70 kDa band observed in Rat Colon, Lung and Spleen lysates (calculated MW of 72.4 kDa according to Rat NP_001102295.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of Jurkat cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concen

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    ITK (IL2-Inducible T-Cell Kinase (ITK))

    别名

    ITK

    背景

    ITK, IL2-inducible T-cell kinase, EMT, LYK, MGC126257, MGC126258, PSCTK2, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, tyrosine-protein kinase LYK

    基因ID

    3702

    NCBI登录号

    NP_005537

    途径

    TCR Signaling, Fc-epsilon Receptor Signaling Pathway
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