FLT1 抗体
Quick Overview for FLT1 抗体 (ABIN1589682)
抗原
See all FLT1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- VEGFR-1/Flt-1 antibody
-
特异性
- Recombinant human soluble Flt-1 (D5)
-
交叉反应 （详细）
- The antibody cross-reacts with mouse Flt-1 (e.g. natural occurring soluble mouse Flt-1) in Western blot analysis.
-
产品特性
-
Chromosomal location: 13q12
The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular Flt-1 D1-D5 as the immunizing antigen. The antibody can be used for ELISA experiments, Western blotting, immunofluorescence, and immunoprecipitation. It will recognize recombinant and naturally occurring form of human VEGFR-1/Flt-1. -
纯化方法
- Mouse IgG1 antibody (clone Flt-1/EWF) from hybridomas was purified from cell culture supernatant by Protein G chromatography.
-
免疫原
- Recombinant human soluble Flt-1 (D5)
-
亚型
- IgG1
-
-
anti-Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (AA 1162-1260) antibody
FLT1 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (AA 20-300) antibodyFLT1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (pTyr1213) antibodyFLT1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (AA 1100-1200) antibodyFLT1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (AA 1-687) antibodyFLT1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) antibodyFLT1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB16736 unconjugated
anti-Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (pTyr1048) antibodyFLT1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (AA 18-308) antibodyFLT1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (AA 1048-1328) antibodyFLT1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (AA 1-380) antibodyFLT1 适用: 人 WB 宿主: 小鼠 Monoclonal 1453CT519-277-79 unconjugated
-
-
应用备注
- ELISA: Use 1-10 μg/mL, Western blotting: Use 1-10 μg/mL, Immunoprecipitation: For IP use 1-5 μg.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
-
缓冲液
- PBS
-
注意事项
- Centrifuge vial prior to opening.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
-
有效期
- 24 months
-
-
- FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))
-
别名
- VEGFR-1/Flt-1
-
背景
- Vascular endothelial growth factor receptor-1, FLT1, VEGF receptor 1,Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVEC supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.
-
基因ID
- 2321
-
NCBI登录号
- NM_001159920, NP_001153392
-
UniProt
- P17948
-
途径
- RTK signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
抗原
-
