VEGFA 抗体
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北京 101111
Quick Overview for VEGFA 抗体 (ABIN155189)
抗原
See all VEGFA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The antibody recognizes VEGF-A under reducing and non-reducing conditions.
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交叉反应 （详细）
- Species reactivity (tested):Human and Mouse.
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纯化方法
- Antigen Affinity Chromatography using recombinant Murine VEGF164 as matrix
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免疫原
- Recombinant Murine VEGF164 Dimer
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anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 28-147) antibody
VEGFA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) antibodyVEGFA 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-214) antibodyVEGFA 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 207-371) antibodyVEGFA 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 6G5B5 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-191) antibodyVEGFA 适用: 人, 大鼠, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 33-48), (N-Term) antibodyVEGFA 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-130) antibodyVEGFA 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3F7 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA 适用: 人 WB, ELISA, Neut 宿主: 山羊 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: To detect murine VEGF-A by direct ELISA a concentration of at least 0.5 μg/mL isrequired. This purified IgG, in combination with compatible secondary reagents, allows thedetection of 0.5-1.0 ng/well of recombinant murine VEGF165 or other VEGF-A splice forms. Western Blot: To detect murine VEGF-A by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions. Immunohistochemistry on Paraffin Sections (Pretreatment: 0.01 % Proteinase K, 15 min atRT).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of > 0.5 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives and stabilizers
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储存液
- Without preservative
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C. DO NOT FREEZE.
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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别名
- VEGF-A
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背景
- VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor
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基因ID
- 7422
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NCBI登录号
- NP_001020537
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UniProt
- P15692
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途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原
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