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Actin 抗体

Cited in 2+ publications. The 小鼠 单克隆 anti-Actin antibody (Clone 337CT30-10-1) (ABIN1536597) specifically detects Actin in WB, IHC (p) 和 FACS. The antibody is reactive with 人 samples.
产品编号 ABIN1536597
发货至: 中国
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Quick Overview for Actin 抗体 (ABIN1536597)

抗原

See all Actin (ACTA1) 抗体
Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

适用

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宿主

  • 93
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小鼠

克隆类型

  • 74
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  • 1
单克隆

标记

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This Actin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

克隆位点

337CT30-10-1
  • 预测反应

    B, C, M, Pig, Rb, Rat

    纯化方法

    This antibody is purified through a protein G column, followed by dialysis against PBS.

    免疫原

    This ACTA1 monoclonal antibody is generated from mouse immunized with ACTA1 recombinant protein.

    亚型

    IgG1 kappa
  • 应用备注

    WB: 1:500-1:1000. WB: 1:1000. IHC-P: 1:10~50. IHC-P: 1:10~50. FC: 1:10~50

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    ACTA1 Antibody can be refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

    有效期

    6 months
  • Vuković, Jevtić, Zhang, Stohr, Levy: "Nuclear size is sensitive to NTF2 protein levels in a manner dependent on Ran binding." in: Journal of cell science, Vol. 129, Issue 6, pp. 1115-27, (2017) (PubMed).

    Edens, Dilsaver, Levy: "PKC-mediated phosphorylation of nuclear lamins at a single serine residue regulates interphase nuclear size in Xenopus and mammalian cells." in: Molecular biology of the cell, Vol. 28, Issue 10, pp. 1389-1399, (2017) (PubMed).

  • 抗原

    Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

    别名

    ACTA1

    背景

    The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

    分子量

    42051

    基因ID

    58

    NCBI登录号

    NP_001091

    UniProt

    P68133

    途径

    Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
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