AKT 1/2/3 抗体 (pTyr315)
Quick Overview for AKT 1/2/3 抗体 (pTyr315) (ABIN1532034)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 281-330, pTyr315
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特异性
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AKT1/2/3 (Phospho-Tyr315/316/312) Antibody detects endogenous levels of AKT1/2/3 only when phosphorylated at Tyr315/316/312.
PhosphorylationH:Y315/316/312 M:Y315/316/312 R:Y315/316/312
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纯化方法
- The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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纯度
- > 95 %
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免疫原
- The antiserum was produced against synthesized peptide derived from human AKT1/2/3 around the phosphorylation site of Tyr315/316/312.
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亚型
- IgG
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anti-AKT 1/2/3 (AA 401-479) antibody
适用: 人, 小鼠, 大鼠, Cow, Pig WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pSer473) antibody适用: 人, 小鼠, 大鼠, Cow, 兔, 小鸡, 鱼 WB, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pSer472) antibody适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 antibody适用: 人, 小鼠, 大鼠, Chimpanzee WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 14E5-16C8-25F6 unconjugated
anti-AKT 1/2/3 (pTyr474) antibody适用: 人, 小鼠 WB, FACS, IF (cc), IF (p), ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibody适用: 人, 小鼠, 大鼠 WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibody适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pThr305), (pThr308), (pThr309) antibody适用: 人, 小鼠, 大鼠 WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (AA 278-312) antibody适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB43841 unconjugated
anti-AKT 1/2/3 (AA 431-480) antibody适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- IHC: 1:50~1:100 ELISA: 1:20000
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说明
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Unigene-Number: Hs.525622, Hs.631535, Hs.498292 (NCBI Gene Symbol: AKT1)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable at -20°C for at least 1 year.
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有效期
- 12 months
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- AKT 1/2/3
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别名
- AKT1/2/3
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背景
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Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT/ RAC-PK-beta, Protein kinase Akt-2, Protein kinase B, beta, PKB beta/ RAC-PK-gamma, Protein kinase Akt-3, Protein kinase B, gamma, PKB gamma, STK-2
NCBI Gene Symbol: AKT1
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分子量
- 55 kDa
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基因ID
- 207, 208, 10000
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OMIM
- 164730
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UniProt
- P31749, P31751, Q9Y243
抗原
-
