HSP27 抗体 (pSer15)
Quick Overview for HSP27 抗体 (pSer15) (ABIN1531866)
抗原
See all HSP27 (HSPB1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 5-54, pSer15
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特异性
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HSP27 (Phospho-Ser15) Antibody detects endogenous levels of HSP27 only when phosphorylated at Ser15.
PhosphorylationH:S15
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纯化方法
- The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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纯度
- > 95 %
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免疫原
- The antiserum was produced against synthesized peptide derived from human HSP27 around the phosphorylation site of Ser15.
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亚型
- IgG
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应用备注
- WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
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说明
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Unigene-Number: Hs.520973 (NCBI Gene Symbol: HSPB1)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable at -20°C for at least 1 year.
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有效期
- 12 months
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- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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别名
- HSP27
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背景
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Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, Heat shock 27 kDa protein, HSP 27, HSP25, HSPB1, P25, SRP27, Stress-responsive protein 27
NCBI Gene Symbol: HSPB1
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分子量
- 22 kDa
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基因ID
- 3315
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OMIM
- 602195
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UniProt
- P04792
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途径
- MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
抗原
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