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PRAS40 抗体 (pSer1, pThr246)

This 兔 多克隆 antibody specifically detects PRAS40 in WB, IHC, ELISA 和 IF. It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN1531296
发货至: 中国
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Quick Overview for PRAS40 抗体 (pSer1, pThr246) (ABIN1531296)

抗原

See all PRAS40 (AKT1S1) 抗体
PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

适用

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人, 小鼠, 大鼠

宿主

  • 103
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兔

克隆类型

  • 61
  • 56
多克隆

标记

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This PRAS40 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
  • 抗原表位

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    AA 207-256, pSer1, pThr246

    原理

    PRAS40 (phospho Thr246) Antibody

    特异性

    Phospho-PRAS40 (T246) Polyclonal Antibody detects endogenous levels of PRAS40 protein only when phosphorylated at T246.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    纯度

    > 95 %

    免疫原

    The antiserum was produced against synthesized peptide derived from human Akt1 S1 around the phosphorylation site of Thr246. AA range:207-256

    亚型

    IgG
  • 应用备注

    WB 1:500 - 1:2000. ELISA: 1:10000.. IF 1:50-200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20°C/1 year

    有效期

    12 months
  • 抗原

    PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

    别名

    Akt1 S1

    背景

    Synonyms: AKT1S1, PRAS40, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate

    Background: AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008],

    Gene Name: AKT1S1

    Protein Name: Proline-rich AKT1 substrate 1

    分子量

    27 kDa

    基因ID

    84335

    OMIM

    610221

    UniProt

    Q96B36

    途径

    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect
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