SIRT1 抗体 (AA 577-590)
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Quick Overview for SIRT1 抗体 (AA 577-590) (ABIN1493902)
抗原
See all SIRT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 577-590
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特异性
- Human SIRT1. This antibody is expected to recognize both reported isoforms (NP_036370.2, NP_001135970.1).
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预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Mouse (93%) Rat (86%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide C-EKPQEVQTSRNVES from an internal region of human SIRT1 (NP_036370.2, NP_001135970.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Mouse (93%), Rat (86%).
Type of Immunogen: Synthetic peptide
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anti-Sirtuin 1 (SIRT1) (AA 101-200) antibody
SIRT1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 1 (SIRT1) (AA 448-747) antibodySIRT1 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 1 (SIRT1) (AA 456-555) antibodySIRT1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 7B7 unconjugated
anti-Sirtuin 1 (SIRT1) (AA 566-601), (C-Term) antibodySIRT1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB50965 unconjugated
anti-Sirtuin 1 (SIRT1) antibodySIRT1 适用: 人 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1F3 unconjugated
anti-Sirtuin 1 (SIRT1) antibodyKO Validated SIRT1 适用: 人 WB, IHC, IP, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 1 (SIRT1) (N-Term) antibodySIRT1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 豚鼠, 马, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 1 (SIRT1) (AA 566-601) antibodySIRT1 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Sirtuin 1 (SIRT1) (AA 456-555) antibodySIRT1 适用: 人 WB, IF, IHC (p), EIA 宿主: 小鼠 Monoclonal 7B7 unconjugated
anti-Sirtuin 1 (SIRT1) (pSer47) antibodySIRT1 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:64000), WB (0.05 - 0.2 μg/mL)
Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 120kD band observed in lysates of cell line Jurkat (calculated MW of 81.7kD according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16,100(19):10794-9., PMID: 12960381). Recommended concentration: 0.05-0.2 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Avoid freeze-thaw cycles.
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- SIRT1 (Sirtuin 1 (SIRT1))
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别名
- SIRT1 / Sirtuin 1
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背景
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Name/Gene ID: SIRT1
Family: ADP-Ribosyltransferase
Synonyms: SIRT1, SIR2-like protein 1, SIR2alpha, SIR2L1, Sirtuin 1, Sir2-like 1, Sirtuin type 1, HSIR2, HSIRT1 -
基因ID
- 23411
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NCBI登录号
- NP_036370, NP_001135970
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UniProt
- Q96EB6
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途径
- MAPK Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling
抗原
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