GFP 抗体 (Biotin)
(5 references)
Quick Overview for GFP 抗体 (Biotin) (ABIN129729)
抗原
See all GFP 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-406-215
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Supplier
- Rockland
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原理
- GFP Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria).
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产品特性
- Synonyms: rabbit anti-GFP antibody biotin conjugation, biotin conjugated rabbit anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
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纯化方法
- Anti-GFP was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
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Immunogen: Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IHC, IP, ICC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FACS, FM, DB 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FACS, FM 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Biotin Conjugated GFP Antibody has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:10,000 - 1:50,000
IF Microscopy Dilution: 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store Anti-GFP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "A Myt1 family transcription factor defines neuronal fate by repressing non-neuronal genes." in: eLife, Vol. 8, (2020) (PubMed).
: "Myeloid Cell-Derived HB-EGF Drives Tissue Recovery After Pancreatitis." in: Cellular and molecular gastroenterology and hepatology, Vol. 8, Issue 2, pp. 173-192, (2019) (PubMed).
: "Dynamic profiling of double-stranded RNA binding proteins." in: Nucleic acids research, Vol. 43, Issue 15, pp. 7566-76, (2015) (PubMed).
: "HIV-1 Tat-induced microgliosis and synaptic damage via interactions between peripheral and central myeloid cells." in: PLoS ONE, Vol. 6, Issue 9, pp. e23915, (2011) (PubMed).
: "ProMAT calibrator: A tool for reducing experimental bias in antibody microarrays." in: Journal of proteome research, Vol. 8, Issue 8, pp. 3937-43, (2009) (PubMed).
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: "A Myt1 family transcription factor defines neuronal fate by repressing non-neuronal genes." in: eLife, Vol. 8, (2020) (PubMed).
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- GFP (Green Fluorescent Protein (GFP))
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别名
- GFP
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背景
- Background: GFP Antibody is produced from Green fluorescent protein which is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
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UniProt
- P42212
抗原
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