Cyclin E2 抗体 (C-Term)
Quick Overview for Cyclin E2 抗体 (C-Term) (ABIN129714)
抗原
See all Cyclin E2 (CCNE2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-971
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Supplier
- Rockland
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原理
- Cyclin E2 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against mouse Cyclin E2 protein.
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产品特性
- Synonyms: rabbit anti-Cyclin E2 antibody, G1/S-specific cyclin-E2, CCNE2
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at the carboxyl terminus of the Cyclin E2 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Cyclin E2 (CCNE2) (C-Term) antibody
CCNE2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin E2 (CCNE2) antibodyCCNE2 适用: 人, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin E2 (CCNE2) (C-Term) antibodyCCNE2 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin E2 (CCNE2) antibodyCCNE2 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin E2 (CCNE2) (AA 255-404) antibodyCCNE2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin E2 (CCNE2) antibodyCCNE2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin E2 (CCNE2) (AA 1-404) antibodyCCNE2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cyclin E2 (CCNE2) (AA 355-404) antibodyCCNE2 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin E2 (CCNE2) (AA 35-84) antibodyCCNE2 适用: 人, Cow, 豚鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to Cyclin E2 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:200 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:20,000 - 1:85,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.17 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Cyclin E2 (CCNE2)
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别名
- Ccne2
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cyclin E was first identified by its ability to rescue growth of yeast deficient in G1 Cyclins, indicating a role in G1 or G1/S transitions. Over-expression of Cyclin E has been observed in a variety of human tumors. Multiple isoforms of Cyclin E are expressed in tumors but not in normal tissues, suggesting a post-transcriptional regulation of Cyclin E. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27Kip1 and p21Cip1. The catalytic activity associated with Cyclin E2 complexes is cell cycle regulated and peaks at the G1/S transition. Unlike Cyclin E1, which is expressed in most proliferating normal and tumor cells, Cyclin E2 levels were low to undetectable in non-transformed cells and increased significantly in tumor-derived cells.
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基因ID
- 12448, 3769612
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UniProt
- Q9Z238
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases
抗原
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