MARK2 抗体 (pThr595)
Quick Overview for MARK2 抗体 (pThr595) (ABIN129661)
抗原
See all MARK2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr595
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Supplier Product No.
- 600-401-895
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Supplier
- Rockland
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原理
- MARK2 phospho T595 Antibody
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交叉反应 （详细）
- MARK2 pT595 affinity purified antibody is directed against the phosphorylated form of human MARK2 at the pT595 residue.
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产品特性
- Synonyms: rabbit anti-MARK2 pT595 antibody, MARK-2, MARK 2, ELKL motif kinase 1, EMK1, EMK-1, MAP/microtubule affinity regulating kinase 2 antibody, PAR1 homolog antibody, Serine/threonine protein kinase MARK2 antibody, Par-1b, Par1b, PAR1 homolog b
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a. The immunogen peptide was phosphorylated at the residue corresponding to T595.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-MAP/microtubule Affinity-Regulating Kinase 2 (MARK2) (AA 401-500) antibody
MARK2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B12 unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 2 (MARK2) (AA 600-630) antibodyMARK2 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 2 (MARK2) (Internal Region) antibodyMARK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 2 (MARK2) (AA 600-630), (C-Term) antibodyMARK2 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB2527-2528 unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 2 (MARK2) (N-Term) antibodyMARK2 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 2 (MARK2) (AA 373-594) antibodyMARK2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 2 (MARK2) antibodyMARK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 2 (MARK2) (AA 1-755) antibodyMARK2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 2 (MARK2) (AA 401-500) antibodyMARK2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3C5 unconjugated
anti-MAP/microtubule Affinity-Regulating Kinase 2 (MARK2) (N-Term) antibodyMARK2 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-MARK2pT595 antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 83 kDa corresponding to MARK2 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:250 - 1:1,000
ELISA Dilution: 1:1,000 - 1:3,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.6 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- MARK2 (MAP/microtubule Affinity-Regulating Kinase 2 (MARK2))
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别名
- MARK2
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背景
- Background: MARK2 pT595 antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. This antibody recognizes a regulatory phosphorylation on a kinase that is critical to regulation of microtubules, cell cycle and immune responses. MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encode two isoforms of the human ser/thr protein kinase EMK1 called MARK2. These isoforms were characterized by the presence of a 162-bp alternative exon that gives rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be co-expressed in a number of selected cell lines and tissue samples. Human MARK2 was shown to be encoded by a single mRNA that is ubiquitously expressed. This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein. Multiple splice variants exist for this protein.
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基因ID
- 2011
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UniProt
- Q7KZI7
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途径
- SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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