HDAC1 抗体 (AA 450-482)
(3 references)
Quick Overview for HDAC1 抗体 (AA 450-482) (ABIN129652)
抗原
See all HDAC1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 450-482
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Supplier Product No.
- 600-401-879
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Supplier
- Rockland
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原理
- HDAC1 (C-terminus) Antibody
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交叉反应 （详细）
- Anti-HDAC-1 antibody is directed against human HDAC-1 protein.
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产品特性
- Synonyms: rabbit anti-HDAC-1 antibody, HDAC1, HDAC 1, HD 1 antibody, histone deacetylase 1 antibody, Histone deacetylase-1, HD1 antibody, RPD3L1, reduced potassium dependency yeast homolog like 1 antibody
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纯化方法
- HDAC-1 antibody was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-HDAC-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 450-482 of Human HDAC-1.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Histone Deacetylase 1 (HDAC1) antibody
KO Validated HDAC1 适用: 人 WB, IF, IP, ICC, IHC (p), ChIP, IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 1 (HDAC1) (AA 1-482) antibodyHDAC1 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 5C11 unconjugated
anti-Histone Deacetylase 1 (HDAC1) (AA 1-482) antibodyHDAC1 适用: 人 WB, IHC, IF, ICC, FACS 宿主: 小鼠 Monoclonal E22 unconjugated
anti-Histone Deacetylase 1 (HDAC1) (AA 1-482) antibodyHDAC1 适用: 人 ELISA, IHC, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 1 (HDAC1) (AA 1-482) antibodyHDAC1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 5A11 unconjugated
anti-Histone Deacetylase 1 (HDAC1) antibodyKO Validated HDAC1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Histone Deacetylase 1 (HDAC1) (AA 207-412) antibodyHDAC1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 1 (HDAC1) (C-Term) antibodyVerified HDAC1 适用: 人, 小鼠 WB, ELISA, IF, ChIP 宿主: 山羊 Polyclonal unconjugated
anti-Histone Deacetylase 1 (HDAC1) (Middle Region) antibodyHDAC1 适用: 人, 小鼠, 大鼠, 兔, Cow, 犬, 豚鼠, 马, 斑马鱼, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 1 (HDAC1) (AA 271-477) antibodyHDAC1 适用: 人, 小鼠 WB, IHC, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-HDAC-1 Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Specific nuclear staining is observed by IHC. Expect bands at 65 kDa in size corresponding to HDAC-1 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:10,000 - 1:50,000
IF Microscopy Dilution: 10 μg / ml
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.33 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Plasma cell differentiation is controlled by multiple cell division-coupled epigenetic programs." in: Nature communications, Vol. 9, Issue 1, pp. 1698, (2018) (PubMed).
: "Tumour-suppressive microRNA-874 contributes to cell proliferation through targeting of histone deacetylase 1 in head and neck squamous cell carcinoma." in: British journal of cancer, Vol. 108, Issue 8, pp. 1648-58, (2013) (PubMed).
: "ICBP90, an E2F-1 target, recruits HDAC1 and binds to methyl-CpG through its SRA domain." in: Oncogene, Vol. 23, Issue 46, pp. 7601-10, (2004) (PubMed).
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: "Plasma cell differentiation is controlled by multiple cell division-coupled epigenetic programs." in: Nature communications, Vol. 9, Issue 1, pp. 1698, (2018) (PubMed).
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- HDAC1 (Histone Deacetylase 1 (HDAC1))
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别名
- HDAC1
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背景
- Background: HDAC-1 antibody recognizes HDAC1 (also known as HD1, histone deacetylase 1, RPD3, RPD3L1) which belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
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基因ID
- 3065, 13128860
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UniProt
- Q13547
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途径
- Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis
抗原
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