LIM Domain Kinase 1 抗体 (AA 620-647)
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- 抗原 See all LIM Domain Kinase 1 (LIMK1) 抗体
- LIM Domain Kinase 1 (LIMK1)
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抗原表位
- AA 620-647
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This LIM Domain Kinase 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-401-855
- Supplier
- Rockland
- 原理
- LIM Kinase Antibody
- 交叉反应 (详细)
- This affinity purified antibody is directed against human LIM kinase protein.
- 产品特性
- Synonyms: rabbit anti-LIM Kinase antibody, LIMK-1, LIM domain kinase 1 antibody, LIMK1 antibody
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal region near aa 620-647 of human LIM kinase protein.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product LIMK1 Primary Antibody
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- 应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 73 kDa in size corresponding to LIM kinase by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:8,000 - 1:36,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- LIM Domain Kinase 1 (LIMK1)
- 别名
- LIMK1 (LIMK1 产品)
- 背景
- Background: LIM Kinase is also known as LIM-domain containing protein kinase, LIMK-1 and LIMK. There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase 1 and LIM kinase 2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development.
- 基因ID
- 3984, 4505001
- UniProt
- P53667
- 途径
- Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events
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