LIM Domain Kinase 1 抗体 (AA 620-647)
Quick Overview for LIM Domain Kinase 1 抗体 (AA 620-647) (ABIN129635)
抗原
See all LIM Domain Kinase 1 (LIMK1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 620-647
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Supplier Product No.
- 600-401-855
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Supplier
- Rockland
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原理
- LIM Kinase Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human LIM kinase protein.
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产品特性
- Synonyms: rabbit anti-LIM Kinase antibody, LIMK-1, LIM domain kinase 1 antibody, LIMK1 antibody
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal region near aa 620-647 of human LIM kinase protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibody
LIMK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Kinase 1 (LIMK1) antibodyLIMK1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 3H6 unconjugated
anti-LIM Domain Kinase 1 (LIMK1) antibodyLIMK1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 6B4 unconjugated
anti-LIM Domain Kinase 1 (LIMK1) (AA 451-550) antibodyLIMK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Kinase 1 (LIMK1) (AA 548-647) antibodyLIMK1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1B2 unconjugated
anti-LIM Domain Kinase 1 (LIMK1) (AA 548-647) antibodyLIMK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A8 unconjugated
anti-LIM Domain Kinase 1 (LIMK1) (AA 548-647) antibodyLIMK1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2E9 unconjugated
anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibodyLIMK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibodyLIMK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Kinase 1 (LIMK1) (AA 1-647) antibodyLIMK1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 73 kDa in size corresponding to LIM kinase by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:8,000 - 1:36,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- LIM Domain Kinase 1 (LIMK1)
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别名
- LIMK1
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背景
- Background: LIM Kinase is also known as LIM-domain containing protein kinase, LIMK-1 and LIMK. There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase 1 and LIM kinase 2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development.
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基因ID
- 3984, 4505001
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UniProt
- P53667
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途径
- Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events
抗原
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