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LIM Domain Kinase 1 抗体 (AA 620-647)

LIMK1 适用: 小鼠 WB, ELISA, FM 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN129635
Supplier Product No.: 600-401-855
发货至: 中国
  • 抗原 See all LIM Domain Kinase 1 (LIMK1) 抗体
    LIM Domain Kinase 1 (LIMK1)
    抗原表位
    • 24
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    AA 620-647
    适用
    • 106
    • 77
    • 72
    • 2
    • 2
    • 1
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    • 1
    小鼠
    宿主
    • 101
    • 20
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    克隆类型
    • 105
    • 19
    多克隆
    标记
    • 58
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    • 2
    This LIM Domain Kinase 1 antibody is un-conjugated
    应用范围
    • 89
    • 48
    • 26
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    • 19
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    • 8
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    Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
    Supplier Product No.
    600-401-855
    Supplier
    Rockland
    原理
    LIM Kinase Antibody
    交叉反应 (详细)
    This affinity purified antibody is directed against human LIM kinase protein.
    产品特性
    Synonyms: rabbit anti-LIM Kinase antibody, LIMK-1, LIM domain kinase 1 antibody, LIMK1 antibody
    纯化方法
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    过滤
    Sterile filtered
    免疫原

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal region near aa 620-647 of human LIM kinase protein.

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product LIMK1 Primary Antibody
  • 应用备注

    Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 73 kDa in size corresponding to LIM kinase by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:8,000 - 1:36,000

    IF Microscopy Dilution: User Optimized

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    LIM Domain Kinase 1 (LIMK1)
    别名
    LIMK1 (LIMK1 产品)
    背景
    Background: LIM Kinase is also known as LIM-domain containing protein kinase, LIMK-1 and LIMK.  There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase 1 and LIM kinase 2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development.
    基因ID
    3984, 4505001
    UniProt
    P53667
    途径
    Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events
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