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- 抗原 See all SPRY4 抗体
- SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))
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抗原表位
- AA 275-299
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SPRY4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 600-401-697
- Supplier
- Rockland
- 原理
- Sprouty-4 Antibody
- 交叉反应 （详细）
- This affinity purified antibody is directed against human Sprouty-4 protein.
- 产品特性
- Synonyms: Rabbit anti-Sprouty4 antibody, Rabbit anti-Sprouty-4 antibody, Sprouty 4, SPRY4, SPRY 4, Spry-4, Protein sprouty homolog 4
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 275-299 of Human Sprouty-4 protein.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product SPRY4 Primary Antibody
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anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) antibody
SPRY4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (Internal Region) antibodySPRY4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (AA 26-56) antibodySPRY4 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB15585 unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (AA 1-299) antibodySPRY4 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (AA 30-210) antibodySPRY4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (Middle Region) antibodySPRY4 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1 μg/mL to 10 μg/mL
Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band (maybe a doublet) ~35 kDa in size corresponding to Sprouty-4 by western blotting in the appropriate cell lysate or extract. Some non-specific banding may be observed using this antibody.
Western Blot Dilution: 1:100 - 1:1,000
ELISA Dilution: 1:3,000 - 1:12,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))
- 别名
- SPRY4 (SPRY4 产品)
- 背景
- Background: Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.
- 基因ID
- 81848
- NCBI登录号
- NP_001120968
- UniProt
- Q9C004
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