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GFP 抗体 (Alkaline Phosphatase (AP))

Name: GFP 抗体 (Alkaline Phosphatase (AP))
SKU: ABIN129567
Availability: OutOfStock

Rockland 600-305-215 GFP 适用: Aequorea victoria WB, ELISA, IHC 宿主: 小鼠 Monoclonal 9F9-F9 Alkaline Phosphatase (AP)

Western Blot of Mouse anti-GFP Antibody Alkaline Phosphatase Conjugated. (GFP 抗体 (Alkaline Phosphatase (AP)))

1 image

产品编号 ABIN129567

Supplier Product No.: 600-305-215

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
168

15

11

9
Aequorea victoria
宿主
63

56

36

13

9

5

1

1
小鼠
Compatible Secondaries
克隆类型
113

67
单克隆
标记
82

11

10

10

5

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to Alkaline Phosphatase (AP)
应用范围
145

76

49

43

30

29

25

24

16

13

13

13

13

11

4

4

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.

600-305-215

Supplier

Rockland

原理

GFP Antibody Alkaline Phosphatase Conjugated

交叉反应（详细）

Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Alkaline Phosphatase, purified and partially purified Green Fluorescent Protein (Aequorea victoria).

产品特性

Synonyms: mouse anti-GFP antibody alkaline phosphatase conjugation, alk phos conjugated mouse anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

纯化方法

GFP Antibody Alkaline Phosphatase Conjugated was prepared from tissue culture supernatant by Protein A affinity chromatography.

过滤

Sterile filtered

免疫原

Immunogen: Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type: Recombinant Protein

克隆位点

9F9-F9

亚型

IgG1 kappa

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应用备注

Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody was tested by western blot and ELISA. It can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP (code # S000-03) or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:2,000 - 1:12,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

有效期

12 months
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

背景

Background: Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

UniProt

P42212