BMI1 抗体 (AA 252-264)
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Quick Overview for BMI1 抗体 (AA 252-264) (ABIN129562)
抗原
See all BMI1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 252-264
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Supplier Product No.
- 600-101-392
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Supplier
- Rockland
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原理
- BMI1 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human Bmi1 protein.
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产品特性
- Synonyms: goat anti-Bmi-1 antibody, Bmi1, B lymphoma Mo MLV insertion region antibody, Bmi 1 antibody, MGC12685 antibody, Murine leukemia viral (bmi 1) oncogene homolog antibody, Oncogene BMI 1 antibody, PCGF 4 antibody
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 252-264 of human Bmi1 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) antibody
BMI1 适用: 人 IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 3E3 unconjugated
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) (AA 120-326) antibodyBMI1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) (AA 142-326) antibodyBMI1 适用: 人, 小鼠 WB, IHC, IF, StM 宿主: 小鼠 Monoclonal BMI1-2823 unconjugated
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) antibodyBMI1 适用: 人, 小鼠 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB22858 unconjugated
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) (AA 1-326) antibodyBMI1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4E10-1C5 unconjugated
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) (AA 142-326) antibodyBMI1 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal BMI1-2689 unconjugated
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) antibodyKD Validated BMI1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB685 unconjugated Recombinant Antibody
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) (AA 142-326) antibodyBMI1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal BMI1-2690 unconjugated
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) (AA 257-286) antibodyBMI1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) (AA 61-160) antibodyBMI1 适用: 人 WB, ELISA, IHC (p), FACS, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA, immunofluorescence, and western blot. A 1:200 dilution is reported to be effective for Immunofluorescence against Methanol fixed cells. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Bmi1 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:30,000
IF Microscopy Dilution: 1:200
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.91 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))
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别名
- BMI1
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背景
- Background: The Bmi-1 oncogene (also known as polycomb group ring finger 4, MGC12685, murine leukemia viral (bmi 1) oncogene homolog, oncogene BMI 1, polycomb complex protein BMI 1 and RNF51) induces telomerase activity and immortalizes human mammary epithelial cells. Bmi-1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. The polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi-1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation.
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基因ID
- 648, 27883842
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UniProt
- P35226
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途径
- Cell Division Cycle, Autophagy
抗原
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