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RAD23A 抗体 (AA 115-140)

This anti-RAD23A antibody is a 山羊 多克隆 antibody detecting RAD23A in WB 和 ELISA. Suitable for 人. This Primary Antibody has been cited in 1 publication.
Rockland
产品编号 ABIN129556
Supplier Product No.: 600-101-291
发货至: 中国

Quick Overview for RAD23A 抗体 (AA 115-140) (ABIN129556)

抗原

See all RAD23A 抗体
RAD23A (RAD23 Homolog A (RAD23A))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This RAD23A antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA
  • 抗原表位

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    AA 115-140

    Supplier Product No.

    600-101-291

    Supplier

    Rockland

    原理

    HR23A Antibody

    交叉反应 (详细)

    Reactivity occurs against human HR23A protein.

    产品特性

    Synonyms: goat anti-HR23A Antibody, hHR23A antibody, MGC111083 antibody, RAD23 homolog A antibody, RAD23 yeast homolog A antibody, RAD23A antibody, UV excision repair protein RAD23 homolog A

    纯化方法

    This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

    过滤

    Sterile filtered

    免疫原

    Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 115-140 of human HR23A protein.

    Immunogen Type: Conjugated Peptide

    亚型

    IgG
  • 应用备注

    Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 60 kDa in size corresponding to HR23A by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:4,000 - 1:16,000

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.2 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • Zhu, Wani, Arab, El-Mahdy, Ray, Wani: "Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites." in: DNA repair, Vol. 8, Issue 2, pp. 262-73, (2009) (PubMed).

  • 抗原

    RAD23A (RAD23 Homolog A (RAD23A))

    别名

    RAD23A

    背景

    Background: HR23A (also known as UV excision repair protein RAD23 homolog A) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

    基因ID

    5886, 4826964

    UniProt

    P54725

    途径

    DNA Damage Repair
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