RFFL 抗体 (AA 1-363)
Quick Overview for RFFL 抗体 (AA 1-363) (ABIN129535)
抗原
See all RFFL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-363
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Supplier Product No.
- 200-401-932
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Supplier
- Rockland
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原理
- RFFL Antibody
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交叉反应 （详细）
- This protein A purified antibody is directed against human RFFL protein.
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产品特性
- Synonyms: rabbit anti-RFFL antibody, Rififylin, RING finger and FYVE-like domain-containing protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like
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纯化方法
- The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-363 of human RFFL protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Ring Finger and FYVE-Like Domain Containing 1 (RFFL) (AA 2-99) antibody
RFFL 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 3A4 unconjugated
anti-Ring Finger and FYVE-Like Domain Containing 1 (RFFL) (AA 1-190) antibodyRFFL 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger and FYVE-Like Domain Containing 1 (RFFL) (AA 265-294), (C-Term) antibodyRFFL 适用: 人 WB 宿主: 兔 Polyclonal RB40306 unconjugated
anti-Ring Finger and FYVE-Like Domain Containing 1 (RFFL) (AA 251-363) antibodyRFFL 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger and FYVE-Like Domain Containing 1 (RFFL) (AA 2-99) antibodyRFFL 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ring Finger and FYVE-Like Domain Containing 1 (RFFL) (N-Term) antibodyRFFL 适用: 人, 小鼠, Cow, 马, 豚鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger and FYVE-Like Domain Containing 1 (RFFL) (AA 287-336) antibodyRFFL 适用: 人, 小鼠, 大鼠, 马, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger and FYVE-Like Domain Containing 1 (RFFL) (AA 1-190) antibodyRFFL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger and FYVE-Like Domain Containing 1 (RFFL) (Middle Region) antibodyRFFL 适用: 人, 小鼠, 大鼠, Cow, 马, 犬, 兔, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:500 - 1:3,000
Application Note: This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to RFFL protein by western blotting in the appropriate tissue or cell lysate or extract. Isoforms 2 and 3 are 39.7 and 36.6 kDa, respectively and should also cross react with this antibody.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:4,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 5.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))
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别名
- RFFL
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). RFFL, also known as Rififylin, RING finger and FYVE-like domain-containing protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like, is a novel modulator of NF-kB activation. RFFL possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RFFL also possesses anti-apoptotic activity and may bind phosphatidylinositol phosphates. RFFL is a membrane bound cytoplasmic protein that is expressed ubiquitously. RFFL can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes and is rapidly degraded after stimulation with TNFSF10, and probably by caspases. Multiple transcript variants have been detected for this protein. Anti-RFFL Antibody is useful for researcher interested in Cancer, Immunology, transcription, and TNF-alpha/NF-kB research.
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基因ID
- 117584, 17432433
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UniProt
- Q8WZ73
抗原
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