Western blotting (0.5-4 μg/mL). Immunoprecipitation (10-20 μg/mL). Immunohistochemistry (10-20 μg/mL). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
PBS containing 50 % Glycerol, 1 % BSA as stabilizers and 0.02 % Thimerosal as preservative.
储存液
Thimerosal (Merthiolate)
注意事项
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the antibody (in aliquots) at -20 °C.
抗原
ERK1/2 (MAPK1/3)
(Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
别名
ERK1 / ERK2
背景
Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.Synonyms: ERK-1/ERK-2, Extracellular signal regulated kinase 1/2, Insulin stimulated MAP2 kinase, MAPK1/MAPK2, Microtubule associated protein 2 kinase, Mitogen activated protein kinase 1/2/3, P42/P44-MAPK