AKT1 抗体 (pSer473)
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北京 101111
Quick Overview for AKT1 抗体 (pSer473) (ABIN126853)
抗原
See all AKT1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer473
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特异性
- Clone 11E6 specifically recognizes PKB phosphorylated at serine 473.
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产品特性
- Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase
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纯化方法
- Subsequent Ultrafiltration and Size Exclusion Chromatography
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组件
- incl. positive Control
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免疫原
- Synthetic phosphopeptide conjugated to KLH. Epitope: phosphoserine 473 P Q F pS Y S ARemarks: Molecular weight of antigen: 60 kDa
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亚型
- IgG1
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anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody
AKT1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 122-443) antibodyAKT1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibodyAKT1 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2E11 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibodyAKT1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer124) antibodyAKT1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibodyAKT1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 3E3 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibodyAKT1 适用: 人 WB, IHC, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-150) antibodyAKT1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1F7E10 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-150) antibodyAKT1 适用: 人, 大鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 1F7G2 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 381-480) antibodyAKT1 适用: 人 ELISA, IP, PLA, RNAi 宿主: 小鼠 Monoclonal 4C3 unconjugated
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应用备注
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ELISA: Use at 0.05 μg/mL. Immunoblotting (Western): 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Provided Positive Control: Cell lysate from pervanadate treated HepG2 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Positive Control: Cell lysate from HepG2 pervanadate treatedFormat: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15min. with pervanadateReconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.
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限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mL2 x PBS / 0.09 % Sodium Azide / PEG and Sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution,aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80 °Cup to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freeze / thaw cycles.
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有效期
- 12 months
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- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
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别名
- AKT1 / PKB
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背景
- The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far, the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway. Activated PKB is phosphorylated at serine 473 (mTOR/Rictor or DNA-PK) and threonine 308 (PDK1).Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
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基因ID
- 207
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UniProt
- P31749
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途径
- PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect
抗原
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